Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion

dc.contributor.authorOrlandi Sardi, Janaina de Cassia
dc.contributor.authorPolaquini, Carlos Roberto [UNESP]
dc.contributor.authorFreires, Irlan Almeida
dc.contributor.authorCarvalho Galvao, Livia Camara de
dc.contributor.authorLazarini, Josy Goldoni
dc.contributor.authorTorrezan, Guilherme Silva [UNESP]
dc.contributor.authorRegasini, Luis Octavio [UNESP]
dc.contributor.authorRosalen, Pedro Luiz
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T17:34:52Z
dc.date.available2018-11-26T17:34:52Z
dc.date.issued2017-06-01
dc.description.abstractPurpose. Staphylococcus aureus infections have contributed to the global healthcare burden, particularly with regard to hospital-acquired meticillin-resistant S. aureus (MRSA) infections. Methodology. This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae. Results. DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 mu mol l(-1), and minimum bactericidal concentration ranging between 69 and 277 mu mol l(-1). It significantly reduced bacterial biofilm survival - by 22-63% (at MIC, 10 x MIC or 100 x MIC) as compared to the 25-42% reduction by vancomycin (P<0.0001) - and severely affected biofilm cell structures, leading to damaged architecture and the formation of amorphous cell clusters. Treatment with DAC (MIC/4) decreased bacterial adhesion to HaCaT keratinocytes from 1 to 5 h (P<0.0001). Finally, DAC demonstrated low toxicity in G. mellonella at its effective anti-biofilm concentrations. Conclusion. These findings open new avenues for the study of this curcumin derivative as an excellent prototype with anti-MRSA activity.en
dc.description.affiliationUniv Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Piracicaba, SP, Brazil
dc.description.affiliationSao Paulo State Univ Julio de Mesquita Filho, Dept Chem & Environm Sci, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ Julio de Mesquita Filho, Dept Chem & Environm Sci, Sao Jose Do Rio Preto, SP, Brazil
dc.format.extent816-824
dc.identifierhttp://dx.doi.org/10.1099/jmm.0.000494
dc.identifier.citationJournal Of Medical Microbiology. London: Microbiology Soc, v. 66, n. 6, p. 816-824, 2017.
dc.identifier.doi10.1099/jmm.0.000494
dc.identifier.fileWOS000403768800016.pdf
dc.identifier.issn0022-2615
dc.identifier.urihttp://hdl.handle.net/11449/162897
dc.identifier.wosWOS:000403768800016
dc.language.isoeng
dc.publisherMicrobiology Soc
dc.relation.ispartofJournal Of Medical Microbiology
dc.relation.ispartofsjr0,914
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdiacetylcurcumin
dc.subjectcurcumin
dc.subjectStaphylococcus aureus
dc.subjectanti-adhesion
dc.subjectantibiofilm
dc.subjecttoxicity
dc.titleAntibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesionen
dc.typeArtigo
dcterms.rightsHolderMicrobiology Soc
unesp.author.orcid0000-0002-3705-0971[7]

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