EFFECTS OF EXOGENOUS PROLINE AND A NATURAL VENTILATION SYSTEM ON THE IN VITRO GROWTH OF ORCHIDS

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dc.contributor.authorSilva, Adriano Bortolotti da
dc.contributor.authorReis, Caroline Oliveira dos
dc.contributor.authorCazetta, Jairo Osvaldo [UNESP]
dc.contributor.authorCarlin, Samira Domingues
dc.contributor.authorCorrea Landgraf, Paulo Roberto
dc.contributor.authorReis, MarinaCunha dos
dc.contributor.institutionUniv Jose do Rosario Vellano UNIFENAS
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUPD
dc.date.accessioned2018-11-26T16:34:48Z
dc.date.available2018-11-26T16:34:48Z
dc.date.issued2016-05-01
dc.description.abstractMicropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g.L-1 of sucrose, solidified with 6 g.L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 +/- 2 degrees C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g center dot L-1). The experiment was carried out in an entirely randomized design consisting of a 2 x 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g center dot L-1) promoted higher dry mass accumulation and better control of water loss by plants.en
dc.description.affiliationUniv Jose do Rosario Vellano UNIFENAS, Fac Agron, Alfenas, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.description.affiliationUPD, Polo Reg Ctr Oeste, Agencia Paulista Tecnol Agronegocios, Jau, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.format.extent619-626
dc.identifier.citationBioscience Journal. Uberlandia: Univ Federal Uberlandia, v. 32, n. 3, p. 619-626, 2016.
dc.identifier.issn1981-3163
dc.identifier.urihttp://hdl.handle.net/11449/161566
dc.identifier.wosWOS:000377189900006
dc.language.isoeng
dc.publisherUniv Federal Uberlandia
dc.relation.ispartofBioscience Journal
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCattleya walkeriana
dc.subjectMicropropagation
dc.subjectAbiotic stress
dc.titleEFFECTS OF EXOGENOUS PROLINE AND A NATURAL VENTILATION SYSTEM ON THE IN VITRO GROWTH OF ORCHIDSen
dc.typeArtigo
dcterms.rightsHolderUniv Federal Uberlandia
unesp.departmentTecnologia - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias