Quantification of fibrosis and mast cells in the tissue response of endodontic sealer irradiated by low-level laser therapy

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dc.contributor.authorCamargo Villela Berbert, Fabio Luiz [UNESP]
dc.contributor.authorSivieri-Araujo, Gustavo
dc.contributor.authorOliveira Ramalho, Lizeti Toledo [UNESP]
dc.contributor.authorLima Pereira, Sanivia Aparecida
dc.contributor.authorRocha Rodrigues, Denise Bertulucci
dc.contributor.authorSivieri de Araujo, Marcelo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Uberaba
dc.date.accessioned2014-05-20T13:46:47Z
dc.date.available2014-05-20T13:46:47Z
dc.date.issued2011-11-01
dc.description.abstractLow-level laser therapy (LLLT) accelerates tissue repair. Mast cells induce the proliferation of fibroblasts and the development of local fibrosis. The objective of this study was to quantify fibrosis rate and mast cells in connective tissue after endodontic sealer zinc oxide and eugenol (ZOE) was implanted and submitted to LLLT, immediately after implant and again 24 h later. Sixty mice were distributed into three groups: GI, GII, and GIII (n = 20). In GI, the tubes filled with Endofill were implanted in the animals and were not irradiated with LLLT. In GII, the tubes containing Endofill were implanted in the animals and then irradiated with red LLLT (InGaAIP) 685-nm wavelength, D=72 J/Cm(2), E = 2 J, T=58 s, P=35 mW, and in GIII, the tubes with Endofill were implanted and irradiated with infrared LLLT (AsGaAl) 830-nm wavelength, D=70 J/Cm(2), E = 2 J, T=40 s, P=50 mW. After 7 days and 30 days, the animals were killed. A series of 6-mu m-thick sections were obtained and stained with Toluidine Blue and Picrosirius and analyzed under a standard light microscope using a polarized light filter for the quantification of fibrosis. The statistics were qualitative and quantitative with a significance of 5%. The irradiation with LLLT did not offer improvement in the fibrosis rate, however, it provided a significant decrease in the concentration of independent mast cells for the period studied.en
dc.description.affiliationSão Paulo State Univ UNESP, Dept Restorat Dent, Discipline Endodont, Dent Sch Araraquara, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dent Sch Araraquara, Dept Morphol, Discipline Histol, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniv Uberaba, Sch Dent, Dept Pathol, Uberaba, MG, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Restorat Dent, Discipline Endodont, Dent Sch Araraquara, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dent Sch Araraquara, Dept Morphol, Discipline Histol, BR-14801903 Araraquara, SP, Brazil
dc.format.extent741-747
dc.identifierhttp://dx.doi.org/10.1007/s10103-010-0797-6
dc.identifier.citationLasers In Medical Science. London: Springer London Ltd, v. 26, n. 6, p. 741-747, 2011.
dc.identifier.doi10.1007/s10103-010-0797-6
dc.identifier.issn0268-8921
dc.identifier.urihttp://hdl.handle.net/11449/16579
dc.identifier.wosWOS:000299093300003
dc.language.isoeng
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEndodonticsen
dc.subjectZinc oxide and eugenol sealeren
dc.subjectLasersen
dc.subjectFibrosisen
dc.subjectMast cellsen
dc.titleQuantification of fibrosis and mast cells in the tissue response of endodontic sealer irradiated by low-level laser therapyen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer London Ltd
unesp.author.orcid0000-0002-0293-2587[4]
unesp.author.orcid0000-0001-5230-0492[1]
unesp.author.orcid0000-0002-8402-7408[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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Artigos - Odontologia Restauradora - FOAR
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