Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs

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dc.contributor.authorSoares, Janir Alves
dc.contributor.authorLeonardo, Mario Roberto [UNESP]
dc.contributor.authorda Silva, Léa Assed Bezerra [UNESP]
dc.contributor.authorTanomaru Filho, Mario [UNESP]
dc.contributor.authorIto, Izabel Yoko
dc.contributor.institutionFederal University of the Valleys of Jequitinhonha and Mucuri
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSchool of Dentistry of Ribeirão Preto
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:21:50Z
dc.date.available2014-05-27T11:21:50Z
dc.date.issued2006-04-01
dc.description.abstractThis study aimed at evaluating the antisepsis of the root canal system (RCS) and periapical region (PR) provided by rotary instrumentation associated with chlorhexidine + calcium hydroxide as intracanal medicament. Chronic periapical lesions were induced in 26 pre-molar roots in two dogs. After microbiological sampling, automatic instrumentation using the Profile system and irrigation with 5.25% sodium hypochlorite solution, with a final rinse of 14.3% EDTA followed by profuse irrigation with physiological saline were carried out in 18 root canals. After drying the canals, a paste based on calcium hydroxide associated with a 2% chlorhexidine digluconate solution was placed inside them. After 21 days, the medication was removed, leaving the root canals empty and coronally sealed. After 96 hours, a final microbiological sample was obtained, followed by histomicrobiological processing by the Brown & Brenn method. Eight untreated root canals represented the control group (C-G). Based on the Mann-Whitney test at a confidence level of 5% (p < 0.05), the procedures of antisepsis used offered significant efficacy (p < 0.05) resulting in 100.0% of the canals free of microorganisms. In the C-G, an elevated incidence of various microbial morphotypes was confirmed in all sites of the RCS, with the presence of microbial colonies in the periapical region. In contrast, the experimental group showed a similar pattern of infection in the RCS, although less intense and a reduced level of periapical infection (p < 0.05). It was concluded that adequate instrumentation followed by the application of calcium hydroxide + chlorhexidine offered significant elimination of microorganisms.en
dc.description.affiliationDepartment of Endodontics Federal University of the Valleys of Jequitinhonha and Mucuri, Minas Gerais
dc.description.affiliationDepartment of Endodontics School of Dentistry of Araraquara São Paulo State University, São Paulo
dc.description.affiliationDepartment of Pediatric Dentistry School of Dentistry of Ribeirão Preto, Ribeirão Preto, SP
dc.description.affiliationDepartment of Microbiology School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, São Paulo
dc.description.affiliationUnespDepartment of Endodontics School of Dentistry of Araraquara São Paulo State University, São Paulo
dc.format.extent120-126
dc.identifierhttp://dx.doi.org/10.1590/S1806-83242006000200006
dc.identifier.citationBrazilian Oral Research, v. 20, n. 2, p. 120-126, 2006.
dc.identifier.doi10.1590/S1806-83242006000200006
dc.identifier.file2-s2.0-33746362924.pdf
dc.identifier.issn1806-8324
dc.identifier.issn1807-3107
dc.identifier.lattes2665211298584751
dc.identifier.scieloS1806-83242006000200006
dc.identifier.scopus2-s2.0-33746362924
dc.identifier.urihttp://hdl.handle.net/11449/68822
dc.language.isoeng
dc.relation.ispartofBrazilian Oral Research
dc.relation.ispartofjcr1.223
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCalcium hydroxide
dc.subjectChlorhexidine
dc.subjectEndodontics
dc.subjectMicrobiology
dc.subjectbone cement
dc.subjectcalcium hydroxide
dc.subjectchlorhexidine
dc.subjecttopical antiinfective agent
dc.subjectanimal
dc.subjectanimal model
dc.subjectdog
dc.subjectdrug combination
dc.subjectendodontics
dc.subjectinstrumentation
dc.subjectmicrobiology
dc.subjecttooth pulp
dc.subjectAnimals
dc.subjectAnti-Infective Agents, Local
dc.subjectBone Cements
dc.subjectCalcium Hydroxide
dc.subjectDental Pulp Cavity
dc.subjectDogs
dc.subjectDrug Combinations
dc.subjectModels, Animal
dc.subjectRoot Canal Preparation
dc.titleEffect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogsen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bor/iaboutj.htm
unesp.author.lattes2665211298584751
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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