Harmful Effects of Pyraclostrobin on the Fat Body and Pericardial Cells of Foragers of Africanized Honey Bee

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dc.contributor.authorInoue, Lais V. B. [UNESP]
dc.contributor.authorDomingues, Caio E. C. [UNESP]
dc.contributor.authorGregorc, Aleš
dc.contributor.authorSilva-Zacarin, Elaine C. M.
dc.contributor.authorMalaspina, Osmar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Maribor
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2023-07-29T13:22:12Z
dc.date.available2023-07-29T13:22:12Z
dc.date.issued2022-09-01
dc.description.abstractManaged honey bees are daily exposed in agricultural settings or wild environments to multiple stressors. Currently, fungicide residues are increasingly present in bees’ pollen and nectar and can harm colonies’ production and survival. Therefore, our study aimed to evaluate the effects of the fungicide pyraclostrobin on the fat body and pericardial cells of Africanized honey bees. The foragers were divided into three experimental treatment groups and two controls: pyraclostrobin 0.125 ng/µL (FG1), 0.025 ng/µL (FG2), 0.005 ng/µL (FG3), untreated control (CTL), and acetone control (CAC). After five days of oral exposure (ad libitum), the bees were dissected and prepared for histopathological and morphometric analysis. The FG1-treated bees showed extensive cytoarchitecture changes in the fat body and pericardial cells, inducing cell death. Bees from the FG2 group showed disarranged oenocytes, peripheral vacuolization, and pyknotic nuclei of pericardial cells, but the cytoarchitecture was not compromised as observed in FG1. Additionally, immune system cells were observed through the fat body in the FG1 group. Bees exposed to FG3 demonstrated only oenocytes vacuolization. A significant decrease in the oenocyte’s surface area for bees exposed to all pyraclostrobin concentrations was observed compared to the CTL and CAC groups. The bees from the FG1 and FG2 treatment groups presented a reduced surface area of pericardial cells compared to the controls and the FG3 group. This study highlighted the harmful effects of fungicide pyraclostrobin concentrations at the individual bee cellular level, potentially harming the colony level on continuous exposure.en
dc.description.affiliationCentro de Estudos de Insetos Sociais (CEIS) Departamento de Biologia Geral e Aplicada Instituto de Biociências (IB) Universidade Estadual Paulista (UNESP)-“Júlio de Mesquita Filho”, SP
dc.description.affiliationFaculty of Agriculture and Life Sciences University of Maribor, Pivola 10
dc.description.affiliationLaboratório de Ecotoxicologia e Análise de Integridade Ambiental (LEIA) Departamento de Biologia (DBio) Universidade Federal de São Carlos (UFSCar), SP
dc.description.affiliationUnespCentro de Estudos de Insetos Sociais (CEIS) Departamento de Biologia Geral e Aplicada Instituto de Biociências (IB) Universidade Estadual Paulista (UNESP)-“Júlio de Mesquita Filho”, SP
dc.identifierhttp://dx.doi.org/10.3390/toxics10090530
dc.identifier.citationToxics, v. 10, n. 9, 2022.
dc.identifier.doi10.3390/toxics10090530
dc.identifier.issn2305-6304
dc.identifier.scopus2-s2.0-85138596930
dc.identifier.urihttp://hdl.handle.net/11449/247656
dc.language.isoeng
dc.relation.ispartofToxics
dc.sourceScopus
dc.subjectApis melliferaL
dc.subjectbiomarkers
dc.subjectmorphophysiology
dc.subjectresidual concentrations
dc.subjectstrobilurin fungicide
dc.titleHarmful Effects of Pyraclostrobin on the Fat Body and Pericardial Cells of Foragers of Africanized Honey Beeen
dc.typeArtigo
unesp.author.orcid0000-0001-8575-2735[2]
unesp.author.orcid0000-0001-7988-6796[3]

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