Acute and chronic effects of the organophosphate malathion on the pancreatic α and β cell viability, cell structure, and voltage-gated K+ currents.

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dc.contributor.authorMartins, J.R.N
dc.contributor.authorLopes, S.
dc.contributor.authorHurtado, H. N.
dc.contributor.authorda Silva, F. N.
dc.contributor.authorVillard, D. R.
dc.contributor.authorTaboga, S. R. [UNESP]
dc.contributor.authorSouza, K.L.A
dc.contributor.authorQuesada, I.
dc.contributor.authorSoriano, S.
dc.contributor.authorRafacho, A.
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.contributor.institutionUniversity of Alicante
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidad Miguel Hernández
dc.contributor.institutionBiomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM)
dc.date.accessioned2023-07-29T13:34:54Z
dc.date.available2023-07-29T13:34:54Z
dc.date.issued2023-03-01
dc.description.abstractStudies indicate that the pesticide malathion may have a role in diabetes. Herein, we determined the effects of different concentrations of malathion on survival, ultrastructure, and electrophysiologic islet cell parameters. Acutely, high concentrations of malathion (0.5 or 1 mM) increased cell death in rat islet cells, while low concentrations (0.1 mM) caused signs of cell damage in pancreatic α and β cells. Exposure of RINm5F cells to malathion for 24 or 48 h confirmed the reduction in β-cell viability at lower concentrations (0.001–100 µM). Chronic exposure of mouse pancreatic α and β cells to 3 nM of malathion led to increased voltage-gated K+ (Kv) currents in α-cells. Our findings show a time and concentration dependency for the malathion effect on the reduction of islet cell viability and indicate that pancreatic α cells are more sensitive to malathion effects on Kv currents and cell death.en
dc.description.affiliationLaboratory of Investigation in Chronic Diseases LIDoC Department of Physiological Sciences Center of Biological Sciences Federal University of Santa Catarina UFSC
dc.description.affiliationGraduate Program in Pharmacology Center of Biological Sciences Federal University of Santa Catarina UFSC
dc.description.affiliationCentral Laboratory of Electron Microscopy LCME PROPESQ Federal University of Santa Catarina UFSC
dc.description.affiliationDepartment of Physiology Genetics and Microbiology University of Alicante
dc.description.affiliationNUMPEX-BIO Campus Duque de Caxias Universidade Federal do Rio de Janeiro (UFRJ) Campus UFRJ Duque de Caxias Prof. Geraldo Cidade
dc.description.affiliationDepartment of Biological Sciences Laboratory of Microscopy and Microanalysis Universidade Estadual Paulista-UNESP
dc.description.affiliationInstituto de Investigación Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE) Universidad Miguel Hernández
dc.description.affiliationBiomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM)
dc.description.affiliationUnespDepartment of Biological Sciences Laboratory of Microscopy and Microanalysis Universidade Estadual Paulista-UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipGeneralitat Valenciana
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCNPq: 304338/2020–3
dc.description.sponsorshipIdGeneralitat Valenciana: PROMETEO/2020/006
dc.identifierhttp://dx.doi.org/10.1016/j.etap.2022.104046
dc.identifier.citationEnvironmental Toxicology and Pharmacology, v. 98.
dc.identifier.doi10.1016/j.etap.2022.104046
dc.identifier.issn1872-7077
dc.identifier.issn1382-6689
dc.identifier.scopus2-s2.0-85145271508
dc.identifier.urihttp://hdl.handle.net/11449/248117
dc.language.isoeng
dc.relation.ispartofEnvironmental Toxicology and Pharmacology
dc.sourceScopus
dc.subjectEndocrine disrupting chemicals
dc.subjectEnvironmental contaminants
dc.subjectHealth concerns
dc.subjectIslets of Langerhans
dc.subjectMorphology
dc.titleAcute and chronic effects of the organophosphate malathion on the pancreatic α and β cell viability, cell structure, and voltage-gated K+ currents.en
dc.typeArtigo

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