Allogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory response

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dc.contributor.authorBrandão, Jaqueline Souza [UNESP]
dc.contributor.authorAlvarenga, Marina Landim [UNESP]
dc.contributor.authorPfeifer, João Pedro Hubbe [UNESP]
dc.contributor.authordos Santos, Vitor Hugo [UNESP]
dc.contributor.authorFonseca-Alves, Carlos Eduardo [UNESP]
dc.contributor.authorRodrigues, Mirian [UNESP]
dc.contributor.authorLaufer-Amorim, Renée [UNESP]
dc.contributor.authorCastillo, José Antonio Lucas [UNESP]
dc.contributor.authorAlves, Ana Liz Garcia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:19:45Z
dc.date.available2018-12-11T17:19:45Z
dc.date.issued2018-06-01
dc.description.abstractThe superficial digital flexor tendon (SDFT) is a structure frequently affected by injuries in high-performance athletic horses, and there are limited therapeutic options. Regenerative medicine has evolved significantly in treating different illnesses. However, understanding the cellular behaviour during mesenchymal stem cell (MSC) transplantation in healthy tissues is not fully known yet. To address the inflammatory response induced by allogeneic MSC transplantation, this study evaluated the local inflammatory response after the application of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) in the equine tendon compared to an autologous transplant and the control group. Eighteen thoracic limbs (TL) in nine animals were divided into three groups and subjected to the application of AT-MSCs in the healthy tendon. In the allogeneic group (Gallog), the animals received an allogeneic AT-MSC application in the TL. The autologous group (Gauto) received an application of autologous cells in the TL, and in the control group (Gcont), phosphate-buffered saline (PBS) was applied. There were no significant differences among the evaluated groups in the physical, morphological, thermography, and ultrasonography analyses. A higher number of CD3-positive lymphocytes was observed in the Gauto group compared to the control (P < 0.05). Additionally, we did not observe different expressions of CD172 and microvascular density among the groups. The allogeneic transplantation of AT-MSCs did not result in an adverse or inflammatory reaction that compromised the use of these cells in this experiment. Their behaviour was similar to that of autologous transplantation.en
dc.description.affiliationDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Distrito de Rubião Júnior s/n
dc.description.affiliationDepartment de Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Distrito de Rubião Júnior s/n
dc.description.affiliationUnespDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Distrito de Rubião Júnior s/n
dc.description.affiliationUnespDepartment de Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Distrito de Rubião Júnior s/n
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 142322/2016-4 GD
dc.description.sponsorshipIdFAPESP: 2013/17880-3
dc.format.extent423-430
dc.identifierhttp://dx.doi.org/10.1016/j.rvsc.2018.03.012
dc.identifier.citationResearch in Veterinary Science, v. 118, p. 423-430.
dc.identifier.doi10.1016/j.rvsc.2018.03.012
dc.identifier.file2-s2.0-85046125366.pdf
dc.identifier.issn1532-2661
dc.identifier.issn0034-5288
dc.identifier.scopus2-s2.0-85046125366
dc.identifier.urihttp://hdl.handle.net/11449/176242
dc.language.isoeng
dc.relation.ispartofResearch in Veterinary Science
dc.relation.ispartofsjr0,593
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAllogeneic
dc.subjectEquine
dc.subjectImmunologic response
dc.subjectInflammation
dc.subjectMesenchymal stem cells
dc.titleAllogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory responseen
dc.typeArtigo
unesp.author.lattes7773733250141398[9]
unesp.author.orcid0000-0002-6702-6139[5]
unesp.author.orcid0000-0001-9092-7819[9]

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Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ
Artigos - Clínica Veterinária - FMVZ