TiO2 nanoparticles added to dense bovine hydroxyapatite bioceramics increase human osteoblast mineralization activity

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dc.contributor.authorFernandes, Paulo Henrique Martins
dc.contributor.authorBordini, Ester Alves Ferreira
dc.contributor.authorCassiano, Fernanda Balestrero
dc.contributor.authorde Azevedo-Silva, Lucas José
dc.contributor.authorFerrairo, Brunna Mota
dc.contributor.authorLisboa-Filho, Paulo Noronha [UNESP]
dc.contributor.authorFortulan, Carlos Alberto
dc.contributor.authorSoares dos Passos, Diana Gabriela
dc.contributor.authorBorges, Ana Flávia Sanches
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T12:28:25Z
dc.date.available2023-07-29T12:28:25Z
dc.date.issued2022-11-01
dc.description.abstractObjectives: This study evaluated the effect of TiO2 nanoparticles + dense hydroxyapatite (HA) on human osteoblast cells (SAOS-2). Methods: Particulate bovine HA powder with or without the addition of either 5 or 8 % TiO2 (HA, HA/TiO2Np5 % or HA/TiO2Np8 %) were pressed into disks (Ø = 12.5 mm; thickness = 1.3 mm) uniaxially (100 MPa) and isostatically (200 MPa/1 min) and sintered at 1300 °C. Y-TZP disks were used as control. The following tests were performed: Scanning Electron Microscopy and Dispersive Energy Spectroscopy (SEM/EDS), Atomic Force Microscopy (AFM), cell viability assay (Alamar Blue-AB) and mineralized matrix deposition (Alizarin Red-AR). AB and AR data were submitted to 2-way ANOVA/Tukey tests and ANOVA/Tukey tests, respectively. Results: SEM revealed that the surface of HA/TiO2Np5% resembles DPBHA surface, but also contains smaller granules. HA/TiO2Np8% characteristics resembles HA/TiO2Np5% surface, but with irregular topography. Y-TZP showed a typical oxide ceramic surface pattern. EDS revealed Ca, O, and P in all samples. C, O, and Zr appeared in Y-TZP samples. AFM data corroborates SEM analysis. AB test revealed excellent cellular viability for HA/TiO2Np5% group. AR test showed that all groups containing TiO2np had more mineralized matrix deposition than all other groups, with statistically differences between HA/TiO2Np8% and HA cultivated in non-osteogenic medium. Culture in osteogenic medium exhibited much more mineralized matrix deposition by TiO2np groups. Significance: In conclusion, the addition of TiO2np showed chemical, superficial, and biological changes in the reinforced materials. HA/TiO2Np5% showed the best results for cell viability and HA/TiO2Np8% for mineralized matrix deposition.en
dc.description.affiliationDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo, SP
dc.description.affiliationDepartment of Prosthodontics and Periodontics Bauru School of Dentistry University of São Paulo, SP
dc.description.affiliationDepartment of Physics School of Sciences São Paulo State University, SP
dc.description.affiliationDepartment of Mechanical Engineering São Carlos School of Engineering University of São Paulo, SP
dc.description.affiliationUnespDepartment of Physics School of Sciences São Paulo State University, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCNPq: 154705/2015–2
dc.description.sponsorshipIdFAPESP: 2013/07296–2
dc.description.sponsorshipIdFAPESP: 2016/15674-5
dc.description.sponsorshipIdFAPESP: 2018/23639–0
dc.format.extente275-e283
dc.identifierhttp://dx.doi.org/10.1016/j.dental.2022.08.007
dc.identifier.citationDental Materials, v. 38, n. 11, p. e275-e283, 2022.
dc.identifier.doi10.1016/j.dental.2022.08.007
dc.identifier.issn0109-5641
dc.identifier.scopus2-s2.0-85138811674
dc.identifier.urihttp://hdl.handle.net/11449/245978
dc.language.isoeng
dc.relation.ispartofDental Materials
dc.sourceScopus
dc.subjectBiocompatible Materials
dc.subjectCeramics
dc.subjectHydroxyapatite
dc.subjectTitanium dioxide
dc.titleTiO2 nanoparticles added to dense bovine hydroxyapatite bioceramics increase human osteoblast mineralization activityen
dc.typeArtigo

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