Parentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers

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dc.contributor.authorAgapito, J.
dc.contributor.authorRodriguez, J.
dc.contributor.authorHerrera-Velit, P.
dc.contributor.authorTimoteo, O.
dc.contributor.authorRojas, P.
dc.contributor.authorBoettcher, P. J.
dc.contributor.authorGarcia, F. [UNESP]
dc.contributor.authorEspinoza, J. R.
dc.contributor.institutionUniv Peruana Cayetano Heredia
dc.contributor.institutionInstituto de Pesquisas Energéticas e Nucleares (IPEN)
dc.contributor.institutionIAEA
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:31:14Z
dc.date.available2014-05-20T15:31:14Z
dc.date.issued2008-04-01
dc.description.abstractThe aim of this study was to assess and apply a microsatellite multiplex system for parentage determination in alpacas. An approach for parentage testing based on 10 microsatellites was evaluated in a population of 329 unrelated alpacas from different geographical zones in Peru. All microsatellite markers, which amplified in two multiplex reactions, were highly polymorphic with a mean of 14.5 alleles per locus (six to 28 alleles per locus) and an average expected heterozygosity (H-E) of 0.8185 (range of 0.698-0.946). The total parentage exclusion probability was 0.999456 for excluding a candidate parent from parentage of an arbitrary offspring, given only the genotype of the offspring, and 0.999991 for excluding a candidate parent from parentage of an arbitrary offspring, given the genotype of the offspring and the other parent. In a case test of parentage assignment, the microsatellite panel assigned 38 (from 45 cases) offspring parentage to 10 sires with LOD scores ranging from 2.19 x 10(+13) to 1.34 x 10(+15) and Delta values ranging from 2.80 x 10(+12) to 1.34 x 10(+15) with an estimated pedigree error rate of 15.5%. The performance of this multiplex panel of markers suggests that it will be useful in parentage testing of alpacas.en
dc.description.affiliationUniv Peruana Cayetano Heredia, Mol Biotechnol Unit, Res & Dev Labs LID, Lima 100, Peru
dc.description.affiliationIPEN, Lima, Peru
dc.description.affiliationIAEA, Joint FAO IAEA Programme Nucl Tech Food & Agr, A-1400 Vienna, Austria
dc.description.affiliationUniv Estadual Paulista Aracatuba, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Aracatuba, São Paulo, Brazil
dc.format.extent201-203
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2052.2007.01697.x
dc.identifier.citationAnimal Genetics. Oxford: Blackwell Publishing, v. 39, n. 2, p. 201-203, 2008.
dc.identifier.doi10.1111/j.1365-2052.2007.01697.x
dc.identifier.issn0268-9146
dc.identifier.urihttp://hdl.handle.net/11449/40429
dc.identifier.wosWOS:000254381100015
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofAnimal Genetics
dc.relation.ispartofjcr1.841
dc.relation.ispartofsjr0,830
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectalpacaen
dc.subjectgenotypingen
dc.subjectmicrosatellitesen
dc.subjectmultiplexen
dc.subjectparentageen
dc.titleParentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markersen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
unesp.author.orcid0000-0003-2055-7554[8]
unesp.author.orcid0000-0001-9134-7322[1]

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