Genetic diversity and SNP's from the chloroplast coding regions of virus-infected cassava

Página do item simplificado
dc.contributor.authorDe Marchi, Bruno Rossitto [UNESP]
dc.contributor.authorKinene, Tonny
dc.contributor.authorKrause-Sakate, Renate [UNESP]
dc.contributor.authorBoykin, Laura M.
dc.contributor.authorNdunguru, Joseph
dc.contributor.authorKehoe, Monica
dc.contributor.authorAteka, Elijah
dc.contributor.authorTairo, Fred
dc.contributor.authorAmisse, Jamisse
dc.contributor.authorSseruwagi, Peter
dc.contributor.institutionUniversity of Florida
dc.contributor.institutionUniversity of Western Australia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMikocheni Agricultural Research Institute
dc.contributor.institutionDiagnostic Laboratory Service
dc.contributor.institutionJomo Kenyatta University of Agriculture and Technology
dc.contributor.institutionMozambique Agricultural Research Institute
dc.date.accessioned2020-12-12T01:21:04Z
dc.date.available2020-12-12T01:21:04Z
dc.date.issued2020-01-01
dc.description.abstractCassava is a staple food crop in sub-Saharan Africa; it is a rich source of carbohydrates and proteins which currently supports livelihoods of more than 800 million people worldwide. However, its continued production is at stake due to vector-transmitted diseases such as Cassava mosaic disease and Cassava brown streak disease. Currently, the management and control of viral diseases in cassava relies mainly on virus-resistant cultivars of cassava. Thus, the discovery of new target genes for plant virus resistance is essential for the development of more cassava varieties by conventional breeding or genetic engineering. The chloroplast is a common target for plant viruses propagation and is also a potential source for discovering new resistant genes for plant breeding. Non-infected and infected cassava leaf samples were obtained from different locations of East Africa in Tanzania, Kenya and Mozambique. RNA extraction followed by cDNA library preparation and Illumina sequencing was performed. Assembling and mapping of the reads were carried out and 33 partial chloroplast genomes were obtained. Bayesian phylogenetic analysis from 55 chloroplast protein-coding genes of a dataset with 39 taxa was performed and the single nucleotide polymorphisms for the chloroplast dataset were identified. Phylogenetic analysis revealed considerable genetic diversity present in chloroplast partial genome among cultivated cassava of East Africa. The results obtained may supplement data of previously selected resistant materials and aid breeding programs to find diversity and achieve resistance for new cassava varieties.en
dc.description.affiliationGulf Coast Research and Education Center University of Florida
dc.description.affiliationSchool of Molecular Sciences Australian Research Council Centre of Excellence in Plant Energy Biology University of Western Australia
dc.description.affiliationDepartment of Plant Protection UNESP - Universidade Estadual Paulista Julio de Mesquita Filho FCA
dc.description.affiliationMikocheni Agricultural Research Institute
dc.description.affiliationDepartment of Primary Industries and Regional Development Diagnostic Laboratory Service
dc.description.affiliationDepartment of Horticulture Jomo Kenyatta University of Agriculture and Technology
dc.description.affiliationMozambique Agricultural Research Institute
dc.description.affiliationUnespDepartment of Plant Protection UNESP - Universidade Estadual Paulista Julio de Mesquita Filho FCA
dc.description.sponsorshipAustralian Government
dc.description.sponsorshipGovernment of Western Australia
dc.identifierhttp://dx.doi.org/10.7717/peerj.8632
dc.identifier.citationPeerJ, v. 2020, n. 3, 2020.
dc.identifier.doi10.7717/peerj.8632
dc.identifier.issn2167-8359
dc.identifier.scopus2-s2.0-85083523293
dc.identifier.urihttp://hdl.handle.net/11449/198749
dc.language.isoeng
dc.relation.ispartofPeerJ
dc.sourceScopus
dc.subjectCassava brown streak disease
dc.subjectCassava mosaic disease
dc.subjectEast Africa
dc.subjectKenya
dc.subjectManihot esculenta
dc.subjectMozambique
dc.subjectTanzania
dc.titleGenetic diversity and SNP's from the chloroplast coding regions of virus-infected cassavaen
dc.typeArtigo

Arquivos

Coleções

Artigos