Comparison of histological and molecular diagnosis of Helicobacter pylori in benign lesions and gastric adenocarcinoma

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dc.contributor.authorGobbo César, Ana Cristina
dc.contributor.authorCury, Patrícia Maluf
dc.contributor.authorMarques Payão, Spencer Luiz
dc.contributor.authorLiberatore, Paula Rahal [UNESP]
dc.contributor.authorSilva, Ana Elizabete [UNESP]
dc.contributor.institutionUniversidade Paulista
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto
dc.contributor.institutionFaculdade de Medicina de Marília
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T20:01:18Z
dc.date.available2022-04-28T20:01:18Z
dc.date.issued2005-01-01
dc.description.abstractHelicobacter pylori colonization is associated with chronic gastritis, peptic ulcers, intestinal metaplasia, adenocarcinoma and lymphoma of the stomach. The objective of this study was to compare the results of the routinely used histology with molecular diagnosis for the detection of H. pylori. Eighty samples from gastric lesions (chronic gastritis, atrophic gastritis, gastric ulcer, and intestinal metaplasia), 18 gastric adenocarcinoma and 10 normal mucosa H. pylori-negative (control) samples were obtained. All samples were examined histologically (hematoxylin-eosin and Giemsa staining), and PCR amplifications of the species-specific antigen gene (H3H4) and urease A gene segment (H5H6) of H. pylori were made, using the human gene CYP1A1 for DNA quality control. In the benign lesion and adenocarcinoma the infection was detected in 43% (42/98) and 71% (70/98) by histological and molecular diagnosis (p=0.0001), respectively. The PCR test detected H. pylori in 27.5% (22/80) of the benign gastric lesions and in 50% (9/18) of the gastric adenocarcinoma cases, the histological diagnosis being negative for this bacterium. About 2.5% of the samples, exclusively from benign lesions and with a positive histological diagnosis, showed negative molecular results for both primers. Statistically significant differences were found between the histological and the molecular method in intestinal metaplasia (p=0.0461) and gastric adenocarcinoma (p=0.0011), due to underdetection of H. pylori by the histological method, which is probably due to the low density of the bacterium as a consequence of the severe atrophy of the gastric mucosa. Our findings suggest that PCR is the more efficient method for the assessment of H. pylori infection, especially in metaplasia and gastric adenocarcinoma.en
dc.description.affiliationUniversidade Paulista, Araçatuba, SP
dc.description.affiliationFaculdade de Medicina de São José do Rio Preto, Sao Jose do Rio Preto, SP
dc.description.affiliationFaculdade de Medicina de Marília, SP
dc.description.affiliationUniversidade Estadual Paulista Departamento de Biologia, Sao Jose do Rio Preto, SP
dc.description.affiliationDepartamento de Biologia Universidade Estadual Paulista, R. Cristovao Colombo, 2265, 15054-000. Sao Jose do Rio Preto, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Departamento de Biologia, Sao Jose do Rio Preto, SP
dc.description.affiliationUnespDepartamento de Biologia Universidade Estadual Paulista, R. Cristovao Colombo, 2265, 15054-000. Sao Jose do Rio Preto, SP
dc.format.extent12-16
dc.identifier.citationBrazilian Journal of Microbiology, v. 36, n. 1, p. 12-16, 2005.
dc.identifier.issn1517-8382
dc.identifier.scopus2-s2.0-25144519614
dc.identifier.urihttp://hdl.handle.net/11449/224597
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Microbiology
dc.sourceScopus
dc.subjectAdenocarcinoma
dc.subjectGastric lesions
dc.subjectHelicobacter pylori
dc.subjectHistological diagnosis
dc.subjectPCR
dc.titleComparison of histological and molecular diagnosis of Helicobacter pylori in benign lesions and gastric adenocarcinomaen
dc.typeArtigo

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