Screening of xylanolytic Aspergillus fumigatus for prebiotic xylooligosaccharide production using bagasse

Página do item simplificado
dc.contributor.authorCarvalho, Ana Flavia Azevedo [UNESP]
dc.contributor.authorDe Oliva Neto, Pedro [UNESP]
dc.contributor.authorDe Almeida, Paula Zaghetto [UNESP]
dc.contributor.authorDa Silva, Juliana Bueno
dc.contributor.authorEscaramboni, Bruna [UNESP]
dc.contributor.authorPastore, Glaucia Maria
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:41:03Z
dc.date.available2018-12-11T16:41:03Z
dc.date.issued2015-01-01
dc.description.abstractSugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (XOS). Some XOS are considered soluble dietary fibre, with low caloric value and prebiotic effect, but they are expensive and not easily available. In a screening of 138 fungi, only nine were shortlisted, and just Aspergillus fumigatus M51 (35.6 U/mL) and A. fumigatus U2370 (28.5 U/mL) were selected as the most significant producers of xylanases. These fungi had low β-xylosidase activity, which is desirable for the production of XOS. The xylanases from Trichoderma reesei CCT 2768, A. fumigatus M51 and A. fumigatus U2370 gave a significantly higher XOS yield, 11.9, 14.7 and 7.9 % respectively, in a 3-hour reaction with hemicellulose from sugarcane bagasse. These enzymes are relatively thermostable at 40-50 °C and can be used in a wide range of pH values. Furthermore, these xylanases produced more prebiotic XOS (xylobiose and xylotriose) when compared with a commercial xylanase. The xylanases from A. fumigatus M51 reached a high level of XOS production (37.6 %) in 48-72 h using hemicellulose extracted from sugarcane bagasse. This yield represents 68.8 kg of prebiotic XOS per metric tonne of cane bagasse. In addition, in a biorefinery, after hemicellulose extraction for XOS production, the residual cellulose could be used for the production of second-generation ethanol.en
dc.description.affiliationDepartment of Food Science School of Food Engineering State University of Campinas (UNICAMP), Rua Monteiro Lobato 80
dc.description.affiliationDepartment of Biological Sciences School of Science and Letters São Paulo State University (UNESP), Rua Dom Antonio 2100
dc.description.affiliationUnespDepartment of Biological Sciences School of Science and Letters São Paulo State University (UNESP), Rua Dom Antonio 2100
dc.format.extent428-435
dc.identifierhttp://dx.doi.org/10.17113/tb.53.04.15.4160
dc.identifier.citationFood Technology and Biotechnology, v. 53, n. 4, p. 428-435, 2015.
dc.identifier.doi10.17113/tb.53.04.15.4160
dc.identifier.issn1334-2606
dc.identifier.issn1330-9862
dc.identifier.scopus2-s2.0-84958163410
dc.identifier.urihttp://hdl.handle.net/11449/168384
dc.language.isoeng
dc.relation.ispartofFood Technology and Biotechnology
dc.relation.ispartofsjr0,365
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBagasse
dc.subjectLignocellulosic materials
dc.subjectXylanases
dc.subjectXylooligosaccharides
dc.titleScreening of xylanolytic Aspergillus fumigatus for prebiotic xylooligosaccharide production using bagasseen
dc.typeArtigo

Arquivos

Coleções

Artigos