Endodontic Chelators Induce Nitric Oxide Expression by Murine-cultured Macrophages

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dc.contributor.authorPappen, Fernanda Geraldes
dc.contributor.authorSouza, Erick Miranda
dc.contributor.authorGiardino, Luciano
dc.contributor.authorCarlos, Iracilda Zepponi [UNESP]
dc.contributor.authorLeonardo, Mario Roberto
dc.contributor.authorLeonardo, Renato de Toledo [UNESP]
dc.contributor.institutionUniversidade Federal de Pelotas (UFPEL)
dc.contributor.institutionUniv Ctr Maranhao
dc.contributor.institutionUniv Brescia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:46:59Z
dc.date.available2014-05-20T13:46:59Z
dc.date.issued2009-06-01
dc.description.abstractIntroduction: Endodontic chelators may extrude to apical tissues during instrumentation activating cellular events on periapical tissues. This study assessed in vitro the expression of nitric oxide (NO) concentrations by murine peritoneal macrophages after contact with MTAD (Dentsply/Tulsa, Tulsa, OK), Tetraclean (Ogna Laboratori Farmaceutici, Muggio, Italy), Smear Clear (Sybron Endo, Orange, CA), and EDTA (Biodinamica, Ibipora, PR, Brazil). Methods: Macrophage cells were obtained from Swiss mice after peritoneal lavage. Chelators were diluted in distilled water obtaining 12 concentrations, and MTT assay identified the concentrations, per group, displaying the highest cell viability (analysis of variance, p < 0.01). Selected concentrations were tested for NO expression using Griess reaction. Culture medium and lipopolysaccharide (LPS) were used as controls. Results: Analysis of variance and Tukey tests showed that all chelators displayed elevated NO concentrations compared with the negative control (p < 0.01). MTAD induced the lowest NO expression, followed by Tetraclean, EDTA, and Smear Clear. No difference was observed between MTAD and Tetraclean (p > 0.01), Tetraclean and EDTA (p > 0.01), and EDTA and Smear Clear (p > 0.01). LPS ranked similar to both EDTA and Smear Clear (p > 0.01). Conclusion: The tested endodontic chelators displayed severe proinflammatory effects on murine-cultured macrophages. Citric acid-based solutions induce lower No release than EDTA-based irrigants. (J Endod 2009;35:824-828)en
dc.description.affiliationUniv Fed Pelotas, Sch Dent, Dept Semiol & Clin, BR-96015300 Pelotas, RS, Brazil
dc.description.affiliationUniv Ctr Maranhao, Post Grad Dept, Sao Luis, MA, Brazil
dc.description.affiliationUniv Brescia, Sch Dent, Dept Periodontol, Brescia, Italy
dc.description.affiliationUNESP, Pharmaceut Sch, Dept Clin Anal, Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent Prevent & Community Dent, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUNESP, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Pharmaceut Sch, Dept Clin Anal, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil
dc.format.extent824-828
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2009.03.002
dc.identifier.citationJournal of Endodontics. New York: Elsevier B.V., v. 35, n. 6, p. 824-828, 2009.
dc.identifier.doi10.1016/j.joen.2009.03.002
dc.identifier.issn0099-2399
dc.identifier.urihttp://hdl.handle.net/11449/16673
dc.identifier.wosWOS:000267176000007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Endodontics
dc.relation.ispartofjcr2.886
dc.relation.ispartofsjr1,585
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectChelatorsen
dc.subjectcytotoxicityen
dc.subjectirrigation solutionsen
dc.subjectmacrophagesen
dc.subjectnitric oxideen
dc.titleEndodontic Chelators Induce Nitric Oxide Expression by Murine-cultured Macrophagesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0003-2970-6683[3]
unesp.author.orcid0000-0003-2074-0834[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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Artigos - Análises Clínicas - FCFAR
Artigos - Odontologia Restauradora - FOAR