Quantitative proteomic profiling of bovine follicular fluid during follicle development

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dc.contributor.authorFerrazza, Rodrigo de Andrade [UNESP]
dc.contributor.authorMogollon Garcia, Henry David [UNESP]
dc.contributor.authorSantos Schmidt, Elizabeth Moreira dos [UNESP]
dc.contributor.authorCarmichael, Monika Mihm
dc.contributor.authorSouza, Fabiana Ferreira de [UNESP]
dc.contributor.authorBurchmore, Richard
dc.contributor.authorSartori, Roberto
dc.contributor.authorEckersall, Peter David
dc.contributor.authorPinheiro Ferreira, Joao Carlos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Glasgow
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-11-26T17:44:59Z
dc.date.available2018-11-26T17:44:59Z
dc.date.issued2017-12-01
dc.description.abstractBovine follicular fluid (FF) constitutes the microenvironment of follicles and includes various biologically active proteins. We performed a study involving 18 healthy nonlactating Holstein cows to determine the protein expression profile of FF at key stages of follicular development. Follicles were individually aspirated in vivo at predeviation (F1 similar to 7.0 mm), deviation (F1 similar to 8.5 mm), postdeviation (F1 similar to 12.0 mm), and preovulatory stages of follicle development, which were confirmed by measurement of follicular estradiol and progesterone concentrations. The FFs from nine cows were selected for proteomic analysis. After albumin depletion, triplicates of pooled FF were reduced, alkylated, and digested with trypsin. The resulting peptides were labeled with TMTsixplex and quantified using liquid chromatography-mass spectrometry/mass spectrometry. A total of 143 proteins were identified and assigned to a variety of biological processes, including response to stimulus and metabolic processes. Twenty-two differentially (P < 0.05) expressed proteins were found between stages indicating intrafollicular changes over development, with expected deviation time critical to modulate the protein expression. For instance, high concentrations of follistatin, inhibin, serglycin, spondin-1, fibrinogen, and anti-testosterone antibody were found during early stages of follicular development. In contrast, apolipoprotein H, alpha-2-macroglobulin, plasminogen, antithrombin-III, and immunoglobulins were increased after deviation. Among the differentially abundant proteins, 19 were found to be associated with steroidogenesis. Pathway analysis identified proteins that were mainly associated with the acute phase response signaling, coagulation system, complement system, liver/retinoid X receptor activation, and biosynthesis of nitric oxide and reactive oxygen. The differentially expressed proteins provide insights into the size-dependent protein changes in the ovarian follicle microenvironment that could influence follicular function. Summary Sentence The protein dynamic changes in ovarian follicle microenvironment during the follicle development are critical for follicular maturation and influence follicular function in cows.en
dc.description.affiliationSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Clin Sci, Botucatu, SP, Brazil
dc.description.affiliationUniv Glasgow, Sch Vet Med, Inst Biodivers Anim Hlth & Comparat Med, Glasgow, Lanark, Scotland
dc.description.affiliationUniv Glasgow, Coll Med Vet & Life Sci, Glasgow Poly Facil, Glasgow, Lanark, Scotland
dc.description.affiliationUniv Sao Paulo, Luiz de Queiroz Coll Agr, Dept Anim Sci, Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Clin Sci, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/18297-7
dc.description.sponsorshipIdFAPESP: 2013/20083-8
dc.description.sponsorshipIdFAPESP: 2014/21257-2
dc.format.extent835-849
dc.identifierhttp://dx.doi.org/10.1093/biolre/iox148
dc.identifier.citationBiology Of Reproduction. Cary: Oxford Univ Press Inc, v. 97, n. 6, p. 835-849, 2017.
dc.identifier.doi10.1093/biolre/iox148
dc.identifier.fileWOS000423988200006.pdf
dc.identifier.issn0006-3363
dc.identifier.urihttp://hdl.handle.net/11449/163793
dc.identifier.wosWOS:000423988200006
dc.language.isoeng
dc.publisherOxford Univ Press Inc
dc.relation.ispartofBiology Of Reproduction
dc.relation.ispartofsjr1,446
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectfolliculogenesis
dc.subjectproteomic
dc.subjectsteroid hormone
dc.subjectmass spectrometry
dc.subjectHolstein cow
dc.titleQuantitative proteomic profiling of bovine follicular fluid during follicle developmenten
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press Inc
unesp.author.orcid0000-0003-0820-5847[2]
unesp.author.orcid0000-0003-1663-9004[6]
unesp.author.orcid0000-0001-6917-0057[7]

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