Efficient isolation and proliferation of human adipose-derived mesenchymal stromal cells in xeno-free conditions

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dc.contributor.authorFuoco, Natalia Langenfeld
dc.contributor.authorde Oliveira, Rafael Guilen
dc.contributor.authorMarcelino, Monica Yonashiro
dc.contributor.authorStessuk, Talita
dc.contributor.authorSakalem, Marna Eliana [UNESP]
dc.contributor.authorMedina, Denis Aloisio Lopes
dc.contributor.authorModotti, Waldir Pereira
dc.contributor.authorForte, Andresa
dc.contributor.authorRibeiro-Paes, João Tadeu [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstitute of Medical-Hospital Service (IAM)
dc.contributor.institutionSão Lucas – Cell Therapy Group
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T01:18:03Z
dc.date.available2020-12-12T01:18:03Z
dc.date.issued2020-04-01
dc.description.abstractClassical methods used for culture of adipose-derived mesenchymal stromal cells (ADSCs) use xenobiotic components, which may present a potential risk for biological contamination and/or elicit immunological reactions. Therefore, the aim of this study was to establish a xeno-free methodology for the isolation and proliferation of human ADSCs (hADSCs). hADSCs were isolated by enzymatic digestion or mechanical dissociation and cultured in the presence of fetal bovine serum or human platelet lysate. Proliferation curves were performed as a function of time from the cell culture and used to calculate the population doubling time. Immunophenotyping and differentiation tests were used to identify and characterize the hADSCs. Human ADSCs isolated and cultured in conventional or xenobiotic-free conditions peaked at different days but achieved similar maximum proliferation. The hADSCs differentiation ability was similar in all groups. The characterization of hADSCs by flow cytometry showed low contamination of the cultures by other cell types. The xenobiotic-free methodology described in this study is a feasible and reproducible alternative for isolation and proliferation of hADSCs. This methodology is in accordance with the recommendations of the National Health Surveillance Agency, which proposes avoidance of xenobiotic products.en
dc.description.affiliationBiotechnology Interunits Post-Graduation Program Biomedical Science Institute University of São Paulo (USP)
dc.description.affiliationInstitute of Medical-Hospital Service (IAM)
dc.description.affiliationSão Lucas – Cell Therapy Group
dc.description.affiliationGenetics and Cell Therapy Laboratory (GenTe Cel) São Paulo State University (Unesp)
dc.description.affiliationLaboratório de Genética e Terapia Celular – GenTe Cel Departamento de Biotecnologia – Unesp, Av. Dom Antonio, 2100
dc.description.affiliationUnespGenetics and Cell Therapy Laboratory (GenTe Cel) São Paulo State University (Unesp)
dc.description.affiliationUnespLaboratório de Genética e Terapia Celular – GenTe Cel Departamento de Biotecnologia – Unesp, Av. Dom Antonio, 2100
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent2475-2486
dc.identifierhttp://dx.doi.org/10.1007/s11033-020-05322-9
dc.identifier.citationMolecular Biology Reports, v. 47, n. 4, p. 2475-2486, 2020.
dc.identifier.doi10.1007/s11033-020-05322-9
dc.identifier.issn1573-4978
dc.identifier.issn0301-4851
dc.identifier.scopus2-s2.0-85081588847
dc.identifier.urihttp://hdl.handle.net/11449/198629
dc.language.isoeng
dc.relation.ispartofMolecular Biology Reports
dc.sourceScopus
dc.subjectAdipose tissue
dc.subjectFetal bovine serum (FBS)
dc.subjectMesenchymal stromal cells
dc.subjectPlatelet lysate
dc.subjectStem cells
dc.titleEfficient isolation and proliferation of human adipose-derived mesenchymal stromal cells in xeno-free conditionsen
dc.typeArtigo
unesp.author.orcid0000-0002-7645-5891[9]

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