Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571

de Oliveira, Luana Paula de Azevedo; de Oliveira, Tiago Felipe; Contiero, Jonas [UNESP]; Cazetta, Márcia Luciana

Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571

Data

2016-07-01

Autores

de Oliveira, Luana Paula de Azevedo

de Oliveira, Tiago Felipe

Contiero, Jonas

Cazetta, Márcia Luciana

Tipo

Artigo

Resumo

The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours.