Effect of dietary iron supplementation on the equine fecal microbiome

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dc.contributor.authorArantes, Julia Assis
dc.contributor.authorBorges, Alexandre Secorun [UNESP]
dc.contributor.authorZakia, Luiza Stachewski
dc.contributor.authorSurette, Michael Gordon
dc.contributor.authorWeese, Jeffrey Scott
dc.contributor.authorCosta, Marcio Carvalho
dc.contributor.authorArroyo, Luis Guillermo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Guelph
dc.contributor.institutionMcMaster University
dc.contributor.institutionUniversity of Montréal
dc.date.accessioned2023-07-29T13:49:45Z
dc.date.available2023-07-29T13:49:45Z
dc.date.issued2023-04-01
dc.description.abstractIron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at-80°C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies.en
dc.description.affiliationDepartment of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo, São Paulo
dc.description.affiliationDepartment of Veterinary Clinical Science College of Veterinary Medicine and Animal Science São Paulo State University, São Paulo
dc.description.affiliationDepartment of Clinical Studies Ontario Veterinary College University of Guelph
dc.description.affiliationDepartment of Medicine Farncombe Family Digestive Health Research Institute McMaster University
dc.description.affiliationDepartment of Biomedicine Veterinary Medicine Faculty University of Montréal
dc.description.affiliationUnespDepartment of Veterinary Clinical Science College of Veterinary Medicine and Animal Science São Paulo State University, São Paulo
dc.format.extent97-104
dc.identifier.citationCanadian Journal of Veterinary Research, v. 87, n. 2, p. 97-104, 2023.
dc.identifier.issn1928-9022
dc.identifier.scopus2-s2.0-85151806935
dc.identifier.urihttp://hdl.handle.net/11449/248647
dc.language.isoeng
dc.relation.ispartofCanadian Journal of Veterinary Research
dc.sourceScopus
dc.titleEffect of dietary iron supplementation on the equine fecal microbiomeen
dc.typeArtigo

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