Hypertension modifies OPG, RANK, and RANKL expression during the dental socket bone healing process in spontaneously hypertensive rats

dc.contributor.authorManrique, Natalia [UNESP]
dc.contributor.authorSilva Pereira, Cassiano Costa [UNESP]
dc.contributor.authorLuvizuto, Eloa Rodrigues [UNESP]
dc.contributor.authorRodriguez Sanchez, Maria Del Pilar [UNESP]
dc.contributor.authorOkamoto, Tetuo
dc.contributor.authorOkamoto, Roberta [UNESP]
dc.contributor.authorSumida, Doris H. [UNESP]
dc.contributor.authorAntoniali, Cristina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-10-21T13:10:31Z
dc.date.available2015-10-21T13:10:31Z
dc.date.issued2015-07-01
dc.description.abstractObjective The aim of this study was to evaluate the dental socket bone healing process by histomorphometric and immunohistochemical analysis of osteoprotegerin (OPG), receptor activator of nuclear factor-kappa beta (RANK), and receptor activator of nuclear factor-kappa beta ligand (RANKL) proteins in spontaneously hypertensive rats (SHR).Materials and methods Under general anesthesia, 25 Wistar rats and 25 SHRs underwent upper right incisor extraction. Rats were euthanized after 7, 14, 21, 28, or 42 days of dental extractions. Histomorphometric and immunohistochemical analyses of OPG, RANK, and RANKL proteins were performed.Results Histomorphometric results showed decreased bone healing and reduced bone trabecular thickness in SHRs. Immunohistochemical reactions showed intense RANKL and RANK immunolabeling at 14 and 28 postoperative days and mild OPG immunolabeling at 7, 14, and 21 days after surgery in SHRs.Conclusion The results of this study show that RANK, RANKL, and OPG immunolabeling was altered in SHRs, and these results are associated with bone healing delay and decreased trabecular thickness in SHRs.Clinical relevance Hypertension alters the expression of RANK, RANKL, and OPG and delays the socket bone healing process. These alterations could influence some dental procedures such as orthodontic treatment and implant placement.en
dc.description.affiliationSacred Heart Univ USC, Grad Res Dept, Bauru, SP, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista, Sch Dent Aracatuba, Dept Pediat &Social Dent, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent Aracatuba, Dept Surg, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent Aracatuba, Integrated Clin, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent Aracatuba, Dept Basic Sci, BR-16015050 Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipUNESP Research Internationalization Program (PROINTER/PROPe-UNESP)
dc.description.sponsorshipIdFAPESP: 2008/01893-0
dc.format.extent1319-1327
dc.identifierhttp://link.springer.com/article/10.1007%2Fs00784-014-1369-0
dc.identifier.citationClinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 19, n. 6, p. 1319-1327, 2015.
dc.identifier.doi10.1007/s00784-014-1369-0
dc.identifier.issn1432-6981
dc.identifier.lattes0169955363968097
dc.identifier.lattes1566928219828056
dc.identifier.urihttp://hdl.handle.net/11449/128511
dc.identifier.wosWOS:000356774100016
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofClinical Oral Investigations
dc.relation.ispartofjcr2.386
dc.relation.ispartofsjr0,986
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectExtraction socket healingen
dc.subjectHistomorphometryen
dc.subjectSpontaneously hypertensive ratsen
dc.subjectOPGen
dc.subjectRANKen
dc.subjectRANKLen
dc.titleHypertension modifies OPG, RANK, and RANKL expression during the dental socket bone healing process in spontaneously hypertensive ratsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes0169955363968097
unesp.author.lattes1566928219828056
unesp.author.orcid0000-0002-4169-2918[4]
unesp.author.orcid0000-0002-0315-6161[8]
unesp.author.orcid0000-0002-6773-6966[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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