Radioautographic study of the seasonal distribution of leukocytes in turtles Phrynops hilarii (Chelonia Chelidae)

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dc.contributor.authorPitol, Dimitrius Leonardo [UNESP]
dc.contributor.authorMardegan Issa, Joao Paulo
dc.contributor.authorCaetano, Flávio Henrique [UNESP]
dc.contributor.authorLunardi, Laurelucia Orive
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:55:43Z
dc.date.available2014-05-20T13:55:43Z
dc.date.issued2008-12-01
dc.description.abstractThe aim of this study was to present a morphological description of the leukocytes of Phrynops hilarii turtles according to the seasonal distribution of these cells and to show their replacement in the blood circulation using a radioautographic method. Five animals of both sexes weighing 600-1200 g were used. The animal's blood was aspirated, smeared on glass slides, and stained with the Romanowsky stain, and 500 cells of each animal were counted during each season. The results obtained were analyzed statistically by analysis of variance followed by the Bonferrom test (NCSS), with the level of significance set at p < 0.05. The radioautographic analysis of turtle blood exposed to 1000 mu Ci of H-3-thymidine and developed after 30 days showed a large number of silver grains incorporated into the cells, except for basophils, with cell renewal occurring every seven days. Quantitative data demonstrated a seasonal influence on the distribution of some leukocyte types, with the following p values: heterophils (p = 0.0007), basophils (p = 0.0002), monocytes (p = 0.0016), eosinophils (p = 0.0073). However, using this statistical method, it was not possible to detect a significant difference related to seasonal influence on lymphocytes (p = 0. 16295) or thrombocytes (p = 0. 1046). Using this experimental animal model, a seasonal influence on the distribution of some leukocyte types was observed, and the radioautographic method revealed a cell renewal system occurring every seven days, except for basophils. (C) 2008 Elsevier Ltd. All rights reserved.en
dc.description.affiliationMol & Cellular Biol UNESP, Biosci Inst, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dent Sch Ribeirao Preto, São Paulo, Brazil
dc.description.affiliationUnespMol & Cellular Biol UNESP, Biosci Inst, São Paulo, Brazil
dc.format.extent1381-1386
dc.identifierhttp://dx.doi.org/10.1016/j.micron.2008.01.021
dc.identifier.citationMicron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 39, n. 8, p. 1381-1386, 2008.
dc.identifier.doi10.1016/j.micron.2008.01.021
dc.identifier.issn0968-4328
dc.identifier.lattes4396826019535898
dc.identifier.urihttp://hdl.handle.net/11449/19950
dc.identifier.wosWOS:000260873600044
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofMicron
dc.relation.ispartofjcr1.728
dc.relation.ispartofsjr0,624
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTurtleen
dc.subjectLeukocytesen
dc.subjectSeasonalen
dc.subjectRadioautographyen
dc.subjectPhrynops hilariien
dc.titleRadioautographic study of the seasonal distribution of leukocytes in turtles Phrynops hilarii (Chelonia Chelidae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.lattes4396826019535898
unesp.author.orcid0000-0002-1056-0795[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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