N-acetylcysteine and vancomycin alone and in combination against staphylococci biofilm

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dc.contributor.authorLeite, Bruna
dc.contributor.authorGomes, Fernanda
dc.contributor.authorMelo, Poliana [UNESP]
dc.contributor.authorSouza, Clovis
dc.contributor.authorTeixeira, Pilar
dc.contributor.authorOliveira, Rosário
dc.contributor.authorPizzolitto, Elisabeth [UNESP]
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversity of Minho
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:58Z
dc.date.available2014-05-27T11:29:58Z
dc.date.issued2013-07-18
dc.description.abstractIntroduction: The ability of staphylococci to produce biofilm is an important virulence mechanism that allows bacteria both to adhere and to live on artificial surfaces and to resist to the host immune factors and antibiotics. Staphylococcal infections have become increasingly difficult to treat due their antibiotic resistance. Therefore, there is a continuous need for new and effective treatment alternatives against staphylococcal infections. The main goal of this study was to test N-acetylcysteine (NAC) and vancomycin alone and in combination against S. epidermidis and S. aureus biofilms. Methods: Biofilms were treated with NAC at minimum inhibitory concentration (MIC) and 10 × MIC concentrations and vancomycin at MIC and peak serum concentrations. Results: The use of NAC 10 × MIC alone showed a significant antibactericidal effect, promoting a 4-5 log10 CFU/ mL reduction in biofilm cells. The combination of NAC 10 × MIC with vancomycin (independently of the concentration used) reduced significantly the number of biofilm cells for all strains evaluated (5-6 log10). Conclusion: N-acetylcysteine associated to vancomycin can be a potential therapeutic strategy in the treatment of infections associated to biofilms of S. epidermidis or S. aureus.en
dc.description.affiliationDepartment of Biotechnology Federal University of São Carlos - UFSCar, Rodovia Washington Luis, Km 235, CEP 13560-000, São Carlos, SP
dc.description.affiliationInstitute for Biotechnology and Bioengineering - IBB University of Minho, Braga
dc.description.affiliationSão Paulo State University - USP, Jaboticabal, SP
dc.description.affiliationSão Paulo State University - UNESP, Rua Expedicionários do Brasil, 1621, CEP 14801-360, Araraquara, SP
dc.description.affiliationUnespSão Paulo State University - USP, Jaboticabal, SP
dc.description.affiliationUnespSão Paulo State University - UNESP, Rua Expedicionários do Brasil, 1621, CEP 14801-360, Araraquara, SP
dc.format.extent184-192
dc.identifierhttp://dx.doi.org/10.4322/rbeb.2013.019
dc.identifier.citationRevista Brasileira de Engenharia Biomedica, v. 29, n. 2, p. 184-192, 2013.
dc.identifier.doi10.4322/rbeb.2013.019
dc.identifier.file2-s2.0-84880094709.pdf
dc.identifier.issn1517-3151
dc.identifier.issn1984-7742
dc.identifier.scopus2-s2.0-84880094709
dc.identifier.urihttp://hdl.handle.net/11449/76008
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Engenharia Biomédica
dc.relation.ispartofsjr0,179
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiofilm
dc.subjectN-acetylcysteine
dc.subjectStaphylococcus aureus
dc.subjectStaphylococcus epidermidis
dc.subjectVancomycin
dc.subjectAntibiotic resistance
dc.subjectArtificial surfaces
dc.subjectMinimum inhibitory concentration
dc.subjectTherapeutic strategy
dc.subjectAntibiotics
dc.subjectBacteria
dc.subjectEnzyme inhibition
dc.subjectBiofilms
dc.titleN-acetylcysteine and vancomycin alone and in combination against staphylococci biofilmen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/rbeb/iaboutj.htm#03
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicas - FCFpt

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