DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria

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dc.contributor.authorKuchler, Ulrike
dc.contributor.authorSantos, Gabriel Mulinari dos [UNESP]
dc.contributor.authorHeimel, Patrick
dc.contributor.authorStaehli, Alexandra
dc.contributor.authorStrauss, Franz Josef
dc.contributor.authorTangl, Stefan
dc.contributor.authorGruber, Reinhard
dc.contributor.institutionMed Univ Vienna
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionLudwig Boltzmann Inst Clin & Expt Traumatol
dc.contributor.institutionAustrian Cluster Tissue Regenerat
dc.contributor.institutionUniv Bern
dc.contributor.institutionUniv Chile
dc.date.accessioned2020-12-10T19:37:36Z
dc.date.available2020-12-10T19:37:36Z
dc.date.issued2019-09-30
dc.description.abstractObjectives Deproteinized bovine bone mineral (DBBM) is not resorbable. However, the behavior of DBBM under inflammatory conditions remains unclear. Aim of the study was therefore to evaluate the resorption of DBBM under local inflammatory conditions in vivo using the calvarial osteolysis model. Methods In thirty adult BALB/c mice, DBBM was implanted into the space between the elevated soft tissue and the calvarial bone. Inflammation was induced either by lipopolysaccharides (LPS) injection or by polyethylene particles (Ceridust) mixed with DBBM. Three modalities were randomly applied (n = 10 each): (a) DBBM alone (control), (b) DBBM + LPS, and (c) DBBM + polyethylene particles (Ceridust). Mice were euthanized on day fourteen, and each calvarium was subjected to histological and mu CT analysis. Primary outcome was the size distribution of the DBBM particles. Secondary outcome was the surface erosion of the calvarial bone. Results Histological and mu CT analysis revealed that the size distribution and the volume of DBBM particles in the augmented site were similar between DBBM alone and the combinations with LPS or polyethylene particles. Moreover, histological evaluation showed no signs of erosions of DBBM particles under inflammatory conditions. mu CT analysis and histology further revealed that LPS and the polyethylene particles, but not the DBBM alone, caused severe erosions of the calvarial bone as indicated by large voids representing the massive compensatory new immature woven bone formation on the endosteal surface. Conclusions Local calvarial bone but not the DBBM particles undergo severe resorption and subsequent new bone formation under inflammatory conditions in a mouse model.en
dc.description.affiliationMed Univ Vienna, Univ Clin Dent, Dept Oral Surg, Vienna, Austria
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Dept Oral Surg, Aracatuba, Brazil
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Integrated Clin, Aracatuba, Brazil
dc.description.affiliationMed Univ Vienna, Univ Clin Dent, Karl Donath Lab, Core Facil Hard Tissue & Biomat Res, Vienna, Austria
dc.description.affiliationMed Univ Vienna, Univ Clin Dent, Dept Oral Biol, Vienna, Austria
dc.description.affiliationLudwig Boltzmann Inst Clin & Expt Traumatol, Vienna, Austria
dc.description.affiliationAustrian Cluster Tissue Regenerat, Vienna, Austria
dc.description.affiliationUniv Bern, Sch Dent Med, Dept Periodontol, Bern, Switzerland
dc.description.affiliationUniv Chile, Sch Dent, Dept Conservat Dent, Santiago, Chile
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Dept Oral Surg, Aracatuba, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Integrated Clin, Aracatuba, Brazil
dc.description.sponsorshipOsteology Foundation
dc.description.sponsorshipAustrian Science Fund
dc.description.sponsorshipIdOsteology Foundation: 14-126
dc.description.sponsorshipIdAustrian Science Fund: 4072-B28
dc.format.extent10-17
dc.identifierhttp://dx.doi.org/10.1111/clr.13538
dc.identifier.citationClinical Oral Implants Research. Hoboken: Wiley, v. 31, n. 1, p. 10-17, 2020.
dc.identifier.doi10.1111/clr.13538
dc.identifier.issn0905-7161
dc.identifier.urihttp://hdl.handle.net/11449/196221
dc.identifier.wosWOS:000488579900001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofClinical Oral Implants Research
dc.sourceWeb of Science
dc.subjectbone regeneration
dc.subjectbone substitutes
dc.subjectcalvaria
dc.subjectinflammation
dc.subjectmice
dc.subjectosteoclasts
dc.titleDBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvariaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.orcid0000-0002-5832-7327[5]
unesp.author.orcid0000-0002-9023-4745[6]

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