Effectiveness of antimicrobial photodynamic therapy mediated by butyl toluidine blue in preventing medication-related osteonecrosis of the jaws in rats

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dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorOlivo, Mariane Bocalon [UNESP]
dc.contributor.authorToro, Luan Felipe [UNESP]
dc.contributor.authorFreire, Jéssica de Oliveira Alvarenga [UNESP]
dc.contributor.authorGanzaroli, Vinícius Franzão [UNESP]
dc.contributor.authorGuiati, Isabella Zacarin [UNESP]
dc.contributor.authorNuernberg, Marta Aparecida Alberton [UNESP]
dc.contributor.authorFranciscon, João Paulo Soares [UNESP]
dc.contributor.authorÂngelo Cintra, Luciano Tavares [UNESP]
dc.contributor.authorGarcia, Valdir Gouveia
dc.contributor.authorWainwright, Mark
dc.contributor.authorTheodoro, Leticia Helena [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionLatin American Institute of Dental Research and Education (ILAPEO)
dc.contributor.institutionLiverpool John Moores University
dc.date.accessioned2023-07-29T15:42:16Z
dc.date.available2023-07-29T15:42:16Z
dc.date.issued2022-12-01
dc.description.abstractBackground: Medication-related osteonecrosis of the jaws (MRONJ) is difficult to treat, therefore, prevention would be the ideal clinical approach. This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT), mediated by butyl toluidine blue (BuTB) in the prevention of MRONJ-like lesions after tooth extraction in rats. Methods: Twenty-eight senescent female rats were distributed in groups: VEH and VEH-aPDT, treated with vehicle, ZOL and ZOL-aPDT, treated with 100 µg/Kg of zoledronate, both treatments every three days over seven weeks. After three weeks from the commencement of treatment, the mandibular first molar was extracted. For the VEH and ZOL groups, no local treatment was performed, while with the VEH-aPDT and ZOL-aPDT groups, photodynamic treatment was carried out at 0, 2, and 4 days after extraction. For aPDT, 500μl of BuTB solution was deposited on the dental extraction site (0.5 mg/ml; 60 s), followed by irradiation with low-level laser (InGaAIP; 660 nm; 35 mW; 74.2 J/cm²; 60 s). After 28 postoperative days, euthanasia was performed. The hemimandibles were processed to: (1) histological analysis of tissue repair; (2) histometric analysis of the percentage of newly formed bone tissue (PNFBT) and percentage of non-vital bone tissue (PNVBT); (3) immunohistochemical analysis for tartrate-resistant acid phosphatase (TRAP). Results: The ZOL and ZOL-aPDT groups showed less TRAP-positive cells when compared with VEH and VEH-aPDT. The ZOL group demonstrated great compromise in the tissue repair process, consistent with MRONJ-like lesions. VEH, VEH-aPDT and ZOL-aPDT presented a favorable tissue repair process. PNFBT in the ZOL group was lower than in the VEH, VEH-aPDT and ZOL-aPDT groups, whereas PNVBT in the ZOL group was higher than in the VEH, VEH-aPDT and ZOL-aPDT groups. Conclusion: aPDT mediated by BuTB prevented the occurrence of MRONJ-like lesions after tooth extraction in rats.en
dc.description.affiliationDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528
dc.description.affiliationDepartment of Diagnostic and Surgery School of Dentistry São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528
dc.description.affiliationInstitute of Biosciences São Paulo State University (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250
dc.description.affiliationDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528
dc.description.affiliationLatin American Institute of Dental Research and Education (ILAPEO), R. Jacarezinho, 656
dc.description.affiliationSchool of Pharmacy and Biomolecular Sciences Liverpool John Moores University, Liverpool L3 3AF
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528
dc.description.affiliationUnespDepartment of Diagnostic and Surgery School of Dentistry São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528
dc.description.affiliationUnespInstitute of Biosciences São Paulo State University (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250
dc.description.affiliationUnespDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: #54170-2019
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2022.103172
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 40.
dc.identifier.doi10.1016/j.pdpdt.2022.103172
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.scopus2-s2.0-85143916885
dc.identifier.urihttp://hdl.handle.net/11449/249473
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.sourceScopus
dc.subjectBisphosphonates
dc.subjectButyl toluidine blue
dc.subjectJaws
dc.subjectOsteonecrosis
dc.subjectPhotodynamic therapy
dc.subjectPrevention
dc.titleEffectiveness of antimicrobial photodynamic therapy mediated by butyl toluidine blue in preventing medication-related osteonecrosis of the jaws in ratsen
dc.typeArtigo
unesp.author.orcid0000-0003-4859-0583[1]

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Artigos - Ciências Básicas - FOA
Artigos - Cirurgia e Clínica Integrada - FOA