The effect of phospholipase A2 from Crotalus durissus collilineatus on Leishmania (Leishmania) amazonensis infection

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dc.contributor.authorPassero, Luiz Felipe Domingues [UNESP]
dc.contributor.authorLaurenti, Marcia Dalastra
dc.contributor.authorTomokane, Thaise Y.
dc.contributor.authorCorbett, Carlos Eduardo P.
dc.contributor.authorToyama, Marcos H. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:12:24Z
dc.date.available2014-05-20T13:12:24Z
dc.date.issued2008-04-01
dc.description.abstractIn this study, the effect of phospholipase A2 (PLA2) derived from Crotalus durissus collilineatus was evaluated in vitro and in vivo on experimental cutaneous leishmaniasis. The promastigote and amastigote forms treated with PLA2 presented increased growth rate. In vivo studies showed that PLA2-treated Leishmania (Leishmania) amazonensis promastigotes increased the size of lesions in BALB/c mice, and histopathological analysis showed numerous necrotic regions presenting a higher density of polymorphonuclear, mononuclear, and amastigote cells. Additionally, infected macrophages treated with PLA2 were able to generate prostaglandin E2 (PGE2). Cytokine quantification showed that the supernatant from infected macrophages presented moderate and high amounts of IL-2 and IL-10, respectively. However, in PLA2-treated infected macrophages, suppression of IL-2 levels occurred, but not of IL-10 levels. Observation also revealed that both the supernatant and lysate of L. (L.) amazonensis promastigotes exhibited PLA2 activity, which, in the presence of dexamethasone, showed no reduction in their activities; while glucocorticoid maintained the ability of promastigote forms to infect macrophages, which presented values similar to controls. In conclusion, the results indicate that PLA2 may be a progression factor for cutaneous leishmaniasis, since the PLA2 effect suppressed IL-2 levels and generated PGE2, an inflammatory lipid mediator.en
dc.description.affiliationUniv Estadual Paulista, Unidade Sao Vicente, BR-01246 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Pathol, Infect Dis Lab LIM 50, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Unidade Sao Vicente, BR-01246 São Paulo, Brazil
dc.format.extent1025-1033
dc.identifierhttp://dx.doi.org/10.1007/s00436-007-0871-6
dc.identifier.citationParasitology Research. New York: Springer, v. 102, n. 5, p. 1025-1033, 2008.
dc.identifier.doi10.1007/s00436-007-0871-6
dc.identifier.issn0932-0113
dc.identifier.lattes7710971038558254
dc.identifier.orcid0000-0002-5986-6381
dc.identifier.urihttp://hdl.handle.net/11449/378
dc.identifier.wosWOS:000254460100029
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofParasitology Research
dc.relation.ispartofjcr2.558
dc.relation.ispartofsjr0,991
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleThe effect of phospholipase A2 from Crotalus durissus collilineatus on Leishmania (Leishmania) amazonensis infectionen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes7710971038558254[1]
unesp.author.orcid0000-0002-5986-6381[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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Artigos - Ciências Biológicas - São Vicente