Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules

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dc.contributor.authorPereira, Melissa Carolina [UNESP]
dc.contributor.authorNodari, Elen Fernanda [UNESP]
dc.contributor.authorde Abreu, Marina Rodrigues [UNESP]
dc.contributor.authorPaiatto, Lisiery Negrini
dc.contributor.authorSimioni, Patrícia Ucelli
dc.contributor.authorCamargo-Mathias, Maria Izabel [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2021-06-25T10:24:08Z
dc.date.available2021-06-25T10:24:08Z
dc.date.issued2021-03-01
dc.description.abstractRhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks.en
dc.description.affiliationDepartment of Biology Institute of Biosciences São Paulo State University – UNESP
dc.description.affiliationDepartment of Genetics Evolution and Bioagents Institute of Biology University of Campinas – UNICAMP
dc.description.affiliationUnespDepartment of Biology Institute of Biosciences São Paulo State University – UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2014/02843-8
dc.description.sponsorshipIdFAPESP: 2015/20745-6
dc.description.sponsorshipIdFAPESP: 2018/2999-9
dc.description.sponsorshipIdFAPESP: 2019/02831-3
dc.description.sponsorshipIdCNPq: 300625/2012-0
dc.format.extent387-398
dc.identifierhttp://dx.doi.org/10.1007/s10493-021-00591-w
dc.identifier.citationExperimental and Applied Acarology, v. 83, n. 3, p. 387-398, 2021.
dc.identifier.doi10.1007/s10493-021-00591-w
dc.identifier.issn1572-9702
dc.identifier.issn0168-8162
dc.identifier.scopus2-s2.0-85101456658
dc.identifier.urihttp://hdl.handle.net/11449/205947
dc.language.isoeng
dc.relation.ispartofExperimental and Applied Acarology
dc.sourceScopus
dc.subjectEctoparasite-host relationships
dc.subjectNitric oxide
dc.subjectSaliva
dc.subjectTicks
dc.titleRhipicephalus sanguineus salivary gland extract as a source of immunomodulatory moleculesen
dc.typeArtigo
unesp.author.orcid0000-0002-9418-8934[6]

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