Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions

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dc.contributor.authorRosa Rodrigues de Souza, Carolina [UNESP]
dc.contributor.authorBergis, Hélène
dc.contributor.authorNg, Patricia
dc.contributor.authorGuillier, Laurent
dc.contributor.authorFélix, Benjamin
dc.contributor.authorLeclercq, Alexandre
dc.contributor.authorGnanou Besse, Nathalie
dc.contributor.institutionEnvironmental and Occupational Health Safety. Laboratory for Food Safety (Anses
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionRisk Assessment Department
dc.contributor.institutionPasteur Institute
dc.date.accessioned2023-07-29T13:12:30Z
dc.date.available2023-07-29T13:12:30Z
dc.date.issued2023-09-01
dc.description.abstractListeria monocytogenes can grow under stressful conditions and contaminate various food categories. Progresss in DNA sequencing-based identification methods, such as multi-locus sequence typing (MLST) now allow for more accurate characterization of pathogens. L. monocytogenes MLST genetic diversity is reflected by the different prevalence of the “clonal complexes” (CCs) in foods or infections. Better understanding of the growth potentials of L. monocytogenes is essential for quantitative risk assessment and efficient detection across CCs genetic diversity. Using optical density measurements taken with an automated spectrophotometer, we compared the maximal growth rate and lag phase of 39 strains from 13 different CCs and various food origins, in 3 broths mimicking stresful food conditions (8 °C, aw 0.95 and pH5) and in ISO Standard enrichment broths (Half Fraser and Fraser). This is important as growth could influence risk through pathogen multiplication in food. Besides, enrichment problems could lead to a lack of detection of some CCs. Despite small differences highlighting natural intraspecific variability, our results show that growth performances of L. monocytogenes strains under the conditions tested in selective and non-selective broth do not appear to be strongly correlated to CCs and cannot explain higher CC “virulence” or prevalence.en
dc.description.affiliationAgence Nationale de Sécurité Sanitaire French Agency for Food Environmental and Occupational Health Safety. Laboratory for Food Safety (Anses, 14 rue Pierre et Marie Curie
dc.description.affiliationSchool of Veterinary Medicine and Animal Science (FMVZ-UNESP)
dc.description.affiliationAgence Nationale de Sécurité Sanitaire Risk Assessment Department
dc.description.affiliationPasteur Institute
dc.description.affiliationUnespSchool of Veterinary Medicine and Animal Science (FMVZ-UNESP)
dc.identifierhttp://dx.doi.org/10.1016/j.fm.2023.104303
dc.identifier.citationFood Microbiology, v. 114.
dc.identifier.doi10.1016/j.fm.2023.104303
dc.identifier.issn1095-9998
dc.identifier.issn0740-0020
dc.identifier.scopus2-s2.0-85158033896
dc.identifier.urihttp://hdl.handle.net/11449/247308
dc.language.isoeng
dc.relation.ispartofFood Microbiology
dc.sourceScopus
dc.subjectClonal complex
dc.subjectGrowth
dc.subjectListeria monocytogenes
dc.subjectMLST
dc.subjectSelective enrichment
dc.titleAssessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditionsen
dc.typeArtigo

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