Potencial imunogênico da Rhipicephalusaquaporina 1 sanguineusde Rhipicephalusem cães(Boophilus)domésticosmicroplus contra o carrapato

Página do item simplificado
dc.contributor.authorÉvora, Patricia Martinez [UNESP]
dc.contributor.authorSanches, Gustavo Seron [UNESP]
dc.contributor.authorGuerrero, Felix David
dc.contributor.authorde León, Adalberto Pérez
dc.contributor.authorBechara, Gervásio Henrique [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUnited States Department of Agriculture – USDA
dc.contributor.institutionPontifícia Universidade Católica do Paraná – PUCPR
dc.date.accessioned2018-12-11T17:11:11Z
dc.date.available2018-12-11T17:11:11Z
dc.date.issued2017-01-01
dc.description.abstractThis study evaluated a recombinant aquaporin 1 protein of Rhipicephalus (Boophilus) microplus (RmAQP1) as antigen in a vaccine against R. sanguineus. Five dogs were immunized with RmAQP1 (10 µg) + adjuvant (Montanide) (G1), and five were inoculated with adjuvant only (G2), three times. Twenty-one days after the last immunization, animals of both groups were challenged with R. sanguineus larvae, nymphs and adults, and their biotic potential was compared. Blood samples were collected before each immunization and every 28 days after the last immunization for 10 weeks. Serum antibody titers (IgG) were assessed by ELISA. We observed that: engorgement period of adult females from G1 was 12% shorter than G2; larvae from G1 had 8.7% longer engorgement period than G2 and weighed 7.2% less; nymphs from G1 had 4.5% shorter engorgement period than G2 and weighed 3.6% less; although the antibody titers increased following the second immunization, they rapidly decreased after the third immunization. Results indicated low immunoprotection of RmAQP1 against adult R. sanguineus ticks, and possible efficacy on larvae and nymphs fed on immunized dogs. Further studies should be performed for a full evaluation of the immunoprotection of RmAQP1 against R. sanguineus infestations in dogs.en
dc.description.affiliationDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – UNESP
dc.description.affiliationKnipling-Bushland U.S. Livestock Insects Research Laboratory Veterinary Pest Genomics Center Agricultural Research Service – ARS United States Department of Agriculture – USDA
dc.description.affiliationEscola de Ciências da Vida Pontifícia Universidade Católica do Paraná – PUCPR
dc.description.affiliationUnespDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – UNESP
dc.description.sponsorshipAmerican Rhododendron Society
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipU.S. Department of Agriculture
dc.description.sponsorshipIdFAPESP: #2013/09792-7
dc.description.sponsorshipIdCNPq: #303112/2013-1
dc.format.extent60-66
dc.identifierhttp://dx.doi.org/10.1590/S1984-29612017005
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 26, n. 1, p. 60-66, 2017.
dc.identifier.doi10.1590/S1984-29612017005
dc.identifier.fileS1984-29612017000100060.pdf
dc.identifier.issn0103-846X
dc.identifier.scieloS1984-29612017000100060
dc.identifier.scopus2-s2.0-85017362777
dc.identifier.urihttp://hdl.handle.net/11449/174452
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAquaporin
dc.subjectDomestic dog
dc.subjectImmunity
dc.subjectRhipicephalus sanguineus
dc.subjectRmAQP1
dc.subjectTicks
dc.titlePotencial imunogênico da Rhipicephalusaquaporina 1 sanguineusde Rhipicephalusem cães(Boophilus)domésticosmicroplus contra o carrapatopt
dc.title.alternativeImmunogenic potential of Rhipicephalus (Boophilus) microplus aquaporin 1 against Rhipicephalus sanguineus in domestic dogsen
dc.typeArtigo
unesp.departmentPatologia Veterinária - FCAVpt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S1984-29612017000100060.pdf
Tamanho:
1.27 MB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias