Transcriptional profiling by RNA sequencing of black pepper (Piper nigrum L.) roots infected by Fusarium solani f. sp. piperis

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dc.contributor.authorMoreira, Edith C. O.
dc.contributor.authorPinheiro, Daniel G. [UNESP]
dc.contributor.authorGordo, Sheila M. C.
dc.contributor.authorRodrigues, Simone M.
dc.contributor.authorPessoa, Elaine
dc.contributor.authorSchaller, Hubert
dc.contributor.authorde Lemos, Oriel F.
dc.contributor.authorSilva, Artur
dc.contributor.authorSchneider, Horacio
dc.contributor.authorSilva, Wilson A.
dc.contributor.authorSampaio, Iracilda
dc.contributor.authorDarnet, Sylvain
dc.contributor.institutionUniversidade Federal do Pará (UFPA)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversité de Strasbourg
dc.date.accessioned2018-12-11T16:49:38Z
dc.date.available2018-12-11T16:49:38Z
dc.date.issued2017-10-01
dc.description.abstractBlack pepper (Piper nigrum L.) is the most traded spice worldwide and therefore has great economic value. One of the major limitations of its production is yield losses in fields due to root rot, a disease caused by Fusarium solani f. sp. piperis. This soil-borne pathogen disseminates rapidly in tropical countries. Biotechnological breeding is often presented as an efficient tool to produce resistant pepper cultivars. An RNA-sequencing experiment was used to take a snapshot of the root transcriptome 60 days after infection by the pathogen. The mapping of 67 million SOLiD single-end reads to the Fusarium genome identified many fungal transcripts coding constitutive proteins and two proteins involved in virulence and conidial formation. The annotation of black pepper transcripts obtained by de novo assembly predicted three proteins restricted to this species. While these transcripts were upregulated in infected roots, the corresponding predicted proteins had no hit in databases. A global analysis of differentially expressed black pepper genes, using terms of gene ontology, has demonstrated an enrichment of genes involved in proteolysis, plastid degradation, cell-wall remodeling and secondary metabolism, consistent with toxicity and necrotrophic fungal interactions that force plants to collaborate at the metabolic level. Detailed descriptions of up- or downregulated genes associated with plant defense suggested several genes implied in the biosynthesis of isoprenoids, especially for an upregulated terpene synthase, a putative geranylgeranyl diphosphate synthase, and a downregulated brassinosteroid signaling component (BAK1). These results provide new clues for investigating further target genes and biotechnological approaches for breeding black pepper resistance to Fusarium.en
dc.description.affiliationGenetics and Molecular Biology Laboratory Coastal Studies Institute Bragança Campus Universidade Federal do Pará
dc.description.affiliationDepartment of Genetics Faculty of Medicine of Ribeirão Preto University of São Paulo, Ribeirão Preto
dc.description.affiliationSchool of Agricultural and Veterinary Sciences São Paulo State University (Unesp), Jaboticabal
dc.description.affiliationBrazilian Agricultural Research Corporation - EMBRAPA Amazônia Oriental
dc.description.affiliationInstituto de Ciências Biológicas Universidade Federal do Pará
dc.description.affiliationPlant Isoprenoid Biology (PIB) Team Institut de Biologie Moléculaire des Plantes du CNRS Université de Strasbourg
dc.description.affiliationLaboratoire International Associé PALMHEAT CNRS/UFPA Direction Europe de la Recherche et Coopération Internationale (DERCI)- Campus, Gérard-Mégie 3, rue Michel-Ange
dc.description.affiliationUnespSchool of Agricultural and Veterinary Sciences São Paulo State University (Unesp), Jaboticabal
dc.identifierhttp://dx.doi.org/10.1007/s11738-017-2538-y
dc.identifier.citationActa Physiologiae Plantarum, v. 39, n. 10, 2017.
dc.identifier.doi10.1007/s11738-017-2538-y
dc.identifier.file2-s2.0-85029759365.pdf
dc.identifier.issn0137-5881
dc.identifier.scopus2-s2.0-85029759365
dc.identifier.urihttp://hdl.handle.net/11449/170180
dc.language.isoeng
dc.relation.ispartofActa Physiologiae Plantarum
dc.relation.ispartofsjr0,574
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDifferential expression
dc.subjectFusarium
dc.subjectPiper nigrum
dc.subjectRNA-seq
dc.subjectRoot rot
dc.titleTranscriptional profiling by RNA sequencing of black pepper (Piper nigrum L.) roots infected by Fusarium solani f. sp. piperisen
dc.typeArtigo
unesp.author.orcid0000-0001-6351-0903[12]

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