Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)

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dc.contributor.authorUtsunomia, Ricardo [UNESP]
dc.contributor.authorZerbinato de Andrade Silva, Duilio Mazzoni [UNESP]
dc.contributor.authorRuiz-Ruano, Francisco J.
dc.contributor.authorAraya-Jaime, Cristian [UNESP]
dc.contributor.authorPansonato-Alves, Jose Carlos [UNESP]
dc.contributor.authorScacchetti, Priscilla Cardim [UNESP]
dc.contributor.authorHashimoto, Diogo Teruo [UNESP]
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.authorTrifonov, Vladmir A.
dc.contributor.authorPorto-Foresti, Fabio [UNESP]
dc.contributor.authorCamacho, Juan Pedro M.
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Granada
dc.contributor.institutionInst Mol & Cellular Biol SB RAS
dc.date.accessioned2018-11-26T16:27:56Z
dc.date.available2018-11-26T16:27:56Z
dc.date.issued2016-03-02
dc.description.abstractB chromosomes constitute a heterogeneous mixture of genomic parasites that are sometimes derived intraspecifically from the standard genome of the host species, but result from interspecific hybridization in other cases. The mode of origin determines the DNA content, with the B chromosomes showing high similarity with the A genome in the first case, but presenting higher similarity with a different species in the second. The characid fish Moenkhausia sanctaefilomenae harbours highly invasive B chromosomes, which are present in all populations analyzed to date in the Parana and Tiete rivers. To investigate the origin of these B chromosomes, we analyzed two natural populations: one carrying B chromosomes and the other lacking them, using a combination of molecular cytogenetic techniques, nucleotide sequence analysis and high-throughput sequencing (Illumina HiSeq2000). Our results showed that i) B chromosomes have not yet reached the Paranapanema River basin; ii) B chromosomes are mitotically unstable; iii) there are two types of B chromosomes, the most frequent of which is lightly C-banded (similar to euchromatin in A chromosomes) (B-1), while the other is darkly C-banded (heterochromatin-like) (B-2); iv) the two B types contain the same tandem repeat DNA sequences (18S ribosomal DNA, H3 histone genes, MS3 and MS7 satellite DNA), with a higher content of 18S rDNA in the heterochromatic variant; v) all of these repetitive DNAs are present together only in the paracentromeric region of autosome pair no. 6, suggesting that the B chromosomes are derived from this A chromosome; vi) the two B chromosome variants show MS3 sequences that are highly divergent from each other and from the 0B genome, although the B-2-derived sequences exhibit higher similarity with the 0B genome (this suggests an independent origin of the two B variants, with the less frequent, B-2 type presumably being younger); and vii) the dN/dS ratio for the H3.2 histone gene is almost 4-6 times higher for B chromosomes than for A chromosome sequences, suggesting that purifying selection is relaxed for the DNA sequences located on the B chromosomes, presumably because they are mostly inactive.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, Brazil
dc.description.affiliationUniv Granada, Dept Genet, Granada, Spain
dc.description.affiliationUniv Estadual Paulista, CAUNESP, Sao Paulo, Brazil
dc.description.affiliationInst Mol & Cellular Biol SB RAS, Novosibirsk, Russia
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, CAUNESP, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 13/00953-8
dc.format.extent20
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0150573
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 11, n. 3, 20 p., 2016.
dc.identifier.doi10.1371/journal.pone.0150573
dc.identifier.fileWOS000371724200103.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/161297
dc.identifier.wosWOS:000371724200103
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleUncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)en
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.orcid0000-0003-4639-5207[4]

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