Effect of Conditioning on the Production of Inulinase by Kluyveromyces marxianus var. bulgaricus through Fed-Batch Fermentation

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Berkeley Electronic Press


The effects of sucrose concentration, pH, temperature and aeration rate on the production of biomass and inulinase (2,1-beta-D-fructan fructanohydrolase, EC were investigated using fed-batch fermentation by Kluyveromyces marxianus var. bulgaricus. The best sucrose concentration for enzyme activity was 10 g L-1, whereas activity was inhibited when the sucrose concentration was 20 g L-1. Enzyme activity was strongly influenced by pH of the culture. pH 5.0 was the most suitable for both enzyme activity and cell growth, achieving 98.04 U mL-1 and 14.05 g L-1, respectively, after 72 hours of cultivation. The highest production of inulinase and biomass was observed at temperatures of 25 degrees C and 30 degrees C, whereas a decrease in activity was observed at 35 degrees C and 40 degrees C. The best aeration rate was 1.0 vvm. The increase in aeration rate or oxygen injection provided no increase in enzyme activity and biomass.



Kluyveromyces marxianus var. bulgaricus, sucrose, inulinase, cellular growth, fed-batch

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International Journal of Food Engineering. Berkeley: Berkeley Electronic Press, v. 4, n. 1, p. 20, 2008.