PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis

Página do item simplificado
dc.contributor.authorRebech, Gabriela Torres [UNESP]
dc.contributor.authorVenturin, Gabriela Lovizutto [UNESP]
dc.contributor.authorSiqueira Ito, Lucas Takeshi [UNESP]
dc.contributor.authorBragato, Jaqueline Poleto [UNESP]
dc.contributor.authorde Carvalho Fonseca, Bianca Stefanini [UNESP]
dc.contributor.authorMelo, Larrissa Martins [UNESP]
dc.contributor.authorCosta, Sidnei Ferro [UNESP]
dc.contributor.authorde Rezende Eugênio, Flávia [UNESP]
dc.contributor.authordos Santos, Paulo Sérgio Patto [UNESP]
dc.contributor.authorde Lima, Valéria Marçal Felix [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T00:59:20Z
dc.date.available2020-12-12T00:59:20Z
dc.date.issued2020-01-01
dc.description.abstractLeishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future.en
dc.description.affiliationSão Paulo State University (UNESP) School of Veterinary Medicine
dc.description.affiliationDepartment of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, Rua Clovis Pestana, 793
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Veterinary Medicine
dc.description.affiliationUnespDepartment of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, Rua Clovis Pestana, 793
dc.identifierhttp://dx.doi.org/10.1016/j.vetimm.2019.109970
dc.identifier.citationVeterinary Immunology and Immunopathology, v. 219.
dc.identifier.doi10.1016/j.vetimm.2019.109970
dc.identifier.issn1873-2534
dc.identifier.issn0165-2427
dc.identifier.scopus2-s2.0-85074711194
dc.identifier.urihttp://hdl.handle.net/11449/198108
dc.language.isoeng
dc.relation.ispartofVeterinary Immunology and Immunopathology
dc.sourceScopus
dc.subjectCD279
dc.subjectCytokines
dc.subjectProtozoa
dc.subjectSpleen leukocytes
dc.titlePD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasisen
dc.typeArtigo
unesp.author.orcid0000-0002-4452-3052[8]

Arquivos

Coleções

Artigos