Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant

Página do item simplificado
dc.contributor.authorChacon, Daisy Sotero
dc.contributor.authorSantos, Marlon Dias Mariano
dc.contributor.authorBonilauri, Bernardo
dc.contributor.authorVilasboa, Johnatan
dc.contributor.authorda Costa, Cibele Tesser
dc.contributor.authorda Silva, Ivanice Bezerra
dc.contributor.authorTorres, Taffarel de Melo
dc.contributor.authorde Araújo, Thiago Ferreira
dc.contributor.authorRoque, Alan de Araújo
dc.contributor.authorPilon, Alan Cesar
dc.contributor.authorSelegatto, Denise Medeiros
dc.contributor.authorFreire, Rafael Teixeira
dc.contributor.authorReginaldo, Fernanda Priscila Santos
dc.contributor.authorVoigt, Eduardo Luiz
dc.contributor.authorZuanazzi, José Angelo Silveira
dc.contributor.authorScortecci, Kátia Castanho
dc.contributor.authorCavalheiro, Alberto José [UNESP]
dc.contributor.authorLopes, Norberto Peporine
dc.contributor.authorFerreira, Leandro De Santis
dc.contributor.authorSantos, Leandro Vieira dos
dc.contributor.authorFontes, Wagner
dc.contributor.authorSousa, Marcelo Valle de
dc.contributor.authorCarvalho, Paulo Costa
dc.contributor.authorFett-Neto, Arthur Germano
dc.contributor.authorGiordani, Raquel Brandt
dc.contributor.institutionFederal University of Rio Grande do Norte (UFRN)
dc.contributor.institutionCarlos Chagas Institute
dc.contributor.institutionStanford University School of Medicine
dc.contributor.institutionFederal University of Rio Grande do Sul
dc.contributor.institutionRural Federal University of the Semiarid
dc.contributor.institutionInstitute for Sustainable Development and Environment
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionStructural and Computational Biology Unit
dc.contributor.institutionBarcelona Institute of Science and Technology
dc.contributor.institutionLeiden University
dc.contributor.institutionFederal University of Rio Grande do Norte
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversity of Brasilia
dc.date.accessioned2023-07-29T14:12:11Z
dc.date.available2023-07-29T14:12:11Z
dc.date.issued2022-09-08
dc.description.abstractErythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh conditions of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the differentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated.en
dc.description.affiliationDepartment of Pharmacy Federal University of Rio Grande do Norte (UFRN), RN
dc.description.affiliationComputational and Structural Proteomics Laboratory Carlos Chagas Institute, PR
dc.description.affiliationStanford Cardiovascular Institute Stanford University School of Medicine
dc.description.affiliationPlant Physiology Laboratory Center for Biotechnology and Department of Botany Federal University of Rio Grande do Sul, RS
dc.description.affiliationBioinformatics Biostatistics and Computer Biology Nucleus Rural Federal University of the Semiarid, RN
dc.description.affiliationInstitute for Sustainable Development and Environment, Dunas Park Herbarium, RN
dc.description.affiliationNPPNS Department of Biomolecular Sciences Faculty of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (FCFRP-USP), SP
dc.description.affiliationZimmermann Group European Molecular Biology Laboratory (EMBL) Structural and Computational Biology Unit
dc.description.affiliationSignal and Information Processing for Sensing Systems Institute for Bioengineering of Catalonia (IBEC) Barcelona Institute of Science and Technology
dc.description.affiliationInstitute of Biology Leiden University
dc.description.affiliationDepartment of Cell Biology and Genetics Center for Biosciences Federal University of Rio Grande do Norte, RN
dc.description.affiliationLaboratory of Pharmacognosy Federal University of Rio Grande do Sul, RS
dc.description.affiliationChemistry Institute São Paulo State University (UNESP), SP
dc.description.affiliationGenetics and Molecular Biology Graduate Program Institute of Biology University of Campinas
dc.description.affiliationLaboratory of Protein Chemistry and Biochemistry Department of Cell Biology University of Brasilia, DF
dc.description.affiliationUnespChemistry Institute São Paulo State University (UNESP), SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipInstituto Nacional de Ciência e Tecnologia em Biodiversidade e Produtos Naturais
dc.description.sponsorshipInstituto Serrapilheira
dc.identifierhttp://dx.doi.org/10.3389/fpls.2022.947558
dc.identifier.citationFrontiers in Plant Science, v. 13.
dc.identifier.doi10.3389/fpls.2022.947558
dc.identifier.issn1664-462X
dc.identifier.scopus2-s2.0-85138522061
dc.identifier.urihttp://hdl.handle.net/11449/249173
dc.language.isoeng
dc.relation.ispartofFrontiers in Plant Science
dc.sourceScopus
dc.subjectCaatinga
dc.subjectErythrina velutina
dc.subjectflavonoids
dc.subjectmolecular network
dc.subjecttranscriptome
dc.titleNon-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody planten
dc.typeArtigo

Arquivos

Coleções

Artigos - Química Orgânica - IQ