Screening and HPLC methods for carotenoids in sweetpotato, cassava and maize for plant breeding trials

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dc.contributor.authorKimura, Mieko
dc.contributor.authorKobori, Cintia N.
dc.contributor.authorRodriguez-Amaya, Delia B.
dc.contributor.authorNestel, Penelope
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionInt Food Policy Res Inst
dc.date.accessioned2014-05-20T15:24:35Z
dc.date.available2014-05-20T15:24:35Z
dc.date.issued2007-01-01
dc.description.abstractAnalytical methods for sweetpotato, cassava and maize were developed. In orange and salmon-fleshed sweetpotatoes, (all-E)-beta-carotene predominated and results of spectrophotometric screening and HPLC quantification did not differ significantly. In yellow-fleshed sweetpotato and cassava, however, spectrophotometric screening overestimated the HPLC values because of the presence of several minor carotenoids. Aside from (all-E)-beta-carotene, Z-isomers were present in cassava in appreciable amounts. For both crops, extraction with acetone or tetrahydrofuran: methanol. (1: 1), using a mortar and pestle or a Polytron homogenizer, gave equivalent results. Rehydration of dry maize at room temperature for 30 min or at 85 degrees C for 5, 10 or 15 min gave equivalent results. Concentrations obtained with the C18 and C30 columns did not differ significantly for zeaxanthin, lutein, beta-cryptoxanthin and beta-carotene in the all-E-configuration, but their Z-isomers were difficult to locate in the chromatogram obtained with the C30 column. Extraction with tetrahydrofuran:methanol (1:1) gave significantly lower results for zeaxanthin and lutein. (c) 2005 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Dept Engn & Tecnol Alimentos, Inst Biociencias Letras & Ciências Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Ciência Alimentos, Fac Engn Alimentos, BR-13083862 Campinas, SP, Brazil
dc.description.affiliationInt Food Policy Res Inst, Washington, DC 20036 USA
dc.description.affiliationUnespUniv Estadual Paulista, Dept Engn & Tecnol Alimentos, Inst Biociencias Letras & Ciências Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent1734-1746
dc.identifierhttp://dx.doi.org/10.1016/j.foodchem.2005.10.020
dc.identifier.citationFood Chemistry. Oxford: Elsevier B.V., v. 100, n. 4, p. 1734-1746, 2007.
dc.identifier.doi10.1016/j.foodchem.2005.10.020
dc.identifier.issn0308-8146
dc.identifier.lattes2298375076173727
dc.identifier.urihttp://hdl.handle.net/11449/35173
dc.identifier.wosWOS:000241000300063
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFood Chemistry
dc.relation.ispartofjcr4.946
dc.relation.ispartofsjr1,793
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectsweetpotatopt
dc.subjectcassavapt
dc.subjectmaizept
dc.subjectanalysispt
dc.subjectcarotenoidspt
dc.subjectprovitamin Apt
dc.titleScreening and HPLC methods for carotenoids in sweetpotato, cassava and maize for plant breeding trialsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes2298375076173727
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Engenharia e Tecnologia de Alimentos - IBILCE