Evaluation of antimutagenic activity and mechanisms of action of beta-glucan from barley, in CHO-k1 and HTC cell lines using the micronucleus test

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dc.contributor.authorOliveira, Rodrigo Juliano
dc.contributor.authorRibeiro, Lucia Regina
dc.contributor.authorda Silva, Ariane Fernanda
dc.contributor.authorMatuo, Renata
dc.contributor.authorMantovani, Mario Sergio
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:54:22Z
dc.date.available2014-05-20T13:54:22Z
dc.date.issued2006-10-01
dc.description.abstractDue to the need to identify new antimutagenic agents and to determine their mechanism of action, the present study examined the mechanism of action of the P-glucan with regard to antimutagenicity using the micronucleus assay in CHO-kl and HTC cell lines. The mutagenicity experiments were performed with three different concentrations of P-glucan (5, 10, and 20 mu g/mL), in wich only the highest dose showed mutagenic activity. In the antimutagenicity experiments, the same concentrations of P-glucan were combined with a mutagenic agent, methylmethane sulfonate, or 2-aminoanthracene, using four different treatment protocols: pre-treatment, simultaneous treatment (simple and with pre-incubation), and post-treatment. The results indicate that the CHO-kl cell line treated with MMS presented a chemopreventive activity for all the doses of P-glucan in the different treatment protocols, except for the lowest dose in post-treatment. When HTC cell line treated with MMS is analysed, a chemopreventive activity can be verified for the highest dose in both pre- and post-treatment. For the simple simultaneous treatment, the three doses demonstrated efficacy, while for the simultaneous treatment with pre-incubation only the intermediate concentration was effective. In HTC treated with 2AA both the lowest dose in the pre-treatment protocol and the post-treatment protocol did not show efficacy in preventing DNA damage. The evaluation of the different protocols and the damage decrease percentages observed suggest that P-glucan has both desmutagenic and bioantimutagenic activity. It is necessary, however, to note that efficacy and mechanism of action are subject to variation when compared the two cell lines, since in HTC, representing a drug-metabolizing system, this substance can show a diminished chemopreventive capacity. (c) 2006 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUEL, Dept Biol Geral, Londrina, PR, Brazil
dc.description.affiliationUNESP, Dept Biol Celular, Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol Celular, Rio Claro, SP, Brazil
dc.format.extent1225-1233
dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2006.04.001
dc.identifier.citationToxicology In Vitro. Oxford: Pergamon-Elsevier B.V., v. 20, n. 7, p. 1225-1233, 2006.
dc.identifier.doi10.1016/j.tiv.2006.04.001
dc.identifier.issn0887-2333
dc.identifier.urihttp://hdl.handle.net/11449/19427
dc.identifier.wosWOS:000241218900019
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology in Vitro
dc.relation.ispartofjcr3.105
dc.relation.ispartofsjr0,931
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbeta-glucanpt
dc.subjectHTC cellspt
dc.subjectCHO-k1 cellspt
dc.subjectmechanism of actionpt
dc.subjectantimutagenesispt
dc.titleEvaluation of antimutagenic activity and mechanisms of action of beta-glucan from barley, in CHO-k1 and HTC cell lines using the micronucleus testen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0001-5268-6508[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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