Cytogenetic analysis of A- and B-chromosomes of Prochilodus lineatus (Teleostei, Prochilodontidae) using different restriction enzyme banding and staining methods

dc.contributor.authorMaistro, E. L.
dc.contributor.authorOliveira, C.
dc.contributor.authorForesti, F.
dc.contributor.institutionUniv Alfenas
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:51:19Z
dc.date.available2014-05-20T13:51:19Z
dc.date.issued2000-01-01
dc.description.abstractDifferent cytogenetic techniques were used to analyse the chromosomes of Prochilodus lineatus with the main objective of comparing the base composition of A- and B-chromosomes. The results of digestion of chromosomes with 10 different restriction endonucleases (REs), silver staining, CMA(3) staining and C-banding indicated the existence of different classes of highly repetitive DNA in the A-set and also suggested the existence of compositional differences between the chromatin of A- and B-chromosomes. The 5-BrdU incorporation technique showed a late replicating pattern in all B-chromosomes and in some heterochromatic pericentromeric regions of A-chromosomes. The cleavage with RE BamHI produced a band pattern in all chromosomes of P. lineatus which permitted the tentative pairing of homologues in the karyotype of this species. We concluded that the combined use of the above techniques can contribute to the correct identification of chromosomes and the karyotypic analysis in fishes. on the basis of the results, some aspects of chromosome structure and the origin of the B-chromosomes in P. lineatus are discussed.en
dc.description.affiliationUniv Alfenas, Inst Farm & Nutr, BR-37130000 Alfenas, MG, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent119-125
dc.identifierhttp://dx.doi.org/10.1023/A:1004063031965
dc.identifier.citationGenetica. Dordrecht: Kluwer Academic Publ, v. 108, n. 2, p. 119-125, 2000.
dc.identifier.doi10.1023/A:1004063031965
dc.identifier.issn0016-6707
dc.identifier.lattes0804793944846367
dc.identifier.urihttp://hdl.handle.net/11449/18330
dc.identifier.wosWOS:000165128000003
dc.language.isoeng
dc.publisherKluwer Academic Publ
dc.relation.ispartofGenetica
dc.relation.ispartofjcr1.366
dc.relation.ispartofsjr0,649
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectB-chromosomespt
dc.subjectchromosome bandingpt
dc.subjectfish cytogeneticspt
dc.subjectheterochromatin differentiationpt
dc.subjectProchilodus lineatuspt
dc.titleCytogenetic analysis of A- and B-chromosomes of Prochilodus lineatus (Teleostei, Prochilodontidae) using different restriction enzyme banding and staining methodsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dcterms.rightsHolderKluwer Academic Publ
unesp.author.lattes0804793944846367
unesp.author.orcid0000-0003-0757-7876[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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