Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii

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dc.contributor.authorVissotto de Paiva Diniz, Pedro Paulo
dc.contributor.authorMaggi, Ricardo Guillermo
dc.contributor.authorSchwartz, Denise Saretta
dc.contributor.authorCadenas, Maria Belen
dc.contributor.authorBradley, Julie Meredith
dc.contributor.authorHegarty, Barbara
dc.contributor.authorBreitschwerdt, Edward Bealmear
dc.contributor.institutionN Carolina State Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:32Z
dc.date.available2014-05-20T15:27:32Z
dc.date.issued2007-09-01
dc.description.abstractThe purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the São Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% ( 4/197) and 1.5% ( 3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer ( ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples ( n = 8) were inoculated into a liquid pre-enrichment growth medium ( BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog ( ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii ( pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs.en
dc.description.affiliationN Carolina State Univ, Sch Vet Med, Ctr Comparat Med & Translat Res, Dept Clin Sci,Intracellular Pathogens Res Lab, Raleigh, NC 27606 USA
dc.description.affiliationUniv Estadual Paulista, FMVZ, Sch Vet Med & Anim Sci, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, FMVZ, Sch Vet Med & Anim Sci, São Paulo, Brazil
dc.format.extent697-710
dc.identifierhttp://dx.doi.org/10.1051/vetres:2007023
dc.identifier.citationVeterinary Research. Les Ulis Cedex A: Edp Sciences S A, v. 38, n. 5, p. 697-710, 2007.
dc.identifier.doi10.1051/vetres:2007023
dc.identifier.fileWOS000249779400004.pdf
dc.identifier.issn0928-4249
dc.identifier.urihttp://hdl.handle.net/11449/37501
dc.identifier.wosWOS:000249779400004
dc.language.isoeng
dc.publisherEdp Sciences S A
dc.relation.ispartofVeterinary Research
dc.relation.ispartofjcr2.903
dc.relation.ispartofsjr1,266
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdogspt
dc.subjectBartonella infectionspt
dc.subjectheart diseasept
dc.subjectculturept
dc.subjectBrazilpt
dc.titleCanine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffiien
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderEdp Sciences S A
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Clínica Veterinária - FMVZ