A multiplex PCR approach for the molecular identification and conservation of the Critically endangered daggernose shark

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dc.contributor.authorNachtigall, Pedro G. [UNESP]
dc.contributor.authorRodrigues-Filho, Luis F.S.
dc.contributor.authorSodré, Davidson C.A.
dc.contributor.authorVallinoto, Marcelo
dc.contributor.authorPinhal, Danillo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Pará (UFPA)
dc.date.accessioned2018-12-11T16:47:48Z
dc.date.available2018-12-11T16:47:48Z
dc.date.issued2017-01-01
dc.description.abstractThe daggernose shark Isogomphodon oxyrhynchus is an inshore tropical species endemic to the coastal waters off northeastern South America with limited distribution and restrictive biological traits. According to the IUCN, this species is Critically Endangered owing to a dramatic population decline over the past 10 yr. Here we implemented a multiplex PCR methodology based on species-specific primers for the precise identification of samples from the daggernose shark (body parts and fins). The effectiveness of the method was verified through application for analysis of unidentified shark samples collected in fish markets and a worldwide inventory of over 57 shark species. This reliable molecular system of identification combines the advantages of low cost and high throughput and may be used locally to monitor the capture and trade of this Critically Endangered shark species.en
dc.description.affiliationDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista - UNESP
dc.description.affiliationInstituto de Estudos Costeiros (IECOS) Universidade Federal do Pará - UFPA
dc.description.affiliationUnespDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista - UNESP
dc.format.extent169-175
dc.identifierhttp://dx.doi.org/10.3354/esr00798
dc.identifier.citationEndangered Species Research, v. 32, n. 1, p. 169-175, 2017.
dc.identifier.doi10.3354/esr00798
dc.identifier.file2-s2.0-85020671585.pdf
dc.identifier.issn1613-4796
dc.identifier.issn1863-5407
dc.identifier.scopus2-s2.0-85020671585
dc.identifier.urihttp://hdl.handle.net/11449/169834
dc.language.isoeng
dc.relation.ispartofEndangered Species Research
dc.relation.ispartofsjr1,188
dc.relation.ispartofsjr1,188
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDNA forensics
dc.subjectGenetic identification
dc.subjectITS2
dc.subjectMultiplex PCR
dc.subjectSharks
dc.titleA multiplex PCR approach for the molecular identification and conservation of the Critically endangered daggernose sharken
dc.typeArtigo

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