Expression of pathogenicity-related genes of Xylella fastidiosa in vitro and in planta

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dc.contributor.authorde Souza, A. A.
dc.contributor.authorTakita, M. A.
dc.contributor.authorPereira, E. O.
dc.contributor.authorColetta, H. D.
dc.contributor.authorMachado, M. A.
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:20:19Z
dc.date.available2014-05-20T15:20:19Z
dc.date.issued2005-04-01
dc.description.abstractXylella fastidiosa is responsible for several economically important plant diseases. It is currently assumed that the symptoms are caused by vascular occlusion due to biofilm formation. Microarray technology was previously used to examine the global gene expression profile of X. filstidiosa freshly isolated from symptomatic plants or after several passages by axenic culture medium, and different pathogenicity profiles have been obtained. In the present study the expression of some pathogenicity-related genes was evaluated in vitro and in planta by RT-PCR. The results suggest that adhesion is important at the beginning of biofilm formation, while the genes related to adaptation are essential for the organism's maintenance in planta. Similar results were observed in vitro mainly for the adhesion genes. The pattern of expression observed suggests that adhesion modulates biofilm formation whereas the expression of some adaptation genes may be related to the environment in which the organism is living.en
dc.description.affiliationCtr APTA Citros Sylvio Moreira, BR-13490970 Cordeiropolis, SP, Brazil
dc.description.affiliationEmbrapa Recursos Genet & Biotechnol, BR-70770900 Brasilia, DF, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.format.extent223-228
dc.identifierhttp://dx.doi.org/10.1007/s00284-004-4447-8
dc.identifier.citationCurrent Microbiology. New York: Springer, v. 50, n. 4, p. 223-228, 2005.
dc.identifier.doi10.1007/s00284-004-4447-8
dc.identifier.issn0343-8651
dc.identifier.urihttp://hdl.handle.net/11449/31651
dc.identifier.wosWOS:000228643400009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCurrent Microbiology
dc.relation.ispartofjcr1.373
dc.relation.ispartofsjr0,562
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleExpression of pathogenicity-related genes of Xylella fastidiosa in vitro and in plantaen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer

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