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Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species

dc.contributor.authorde Oliveira Zoccolotti, Jacqueline [UNESP]
dc.contributor.authorCavalheiro, Alberto José [UNESP]
dc.contributor.authorTasso, Camilla Olga [UNESP]
dc.contributor.authorRibas, Beatriz Ribeiro [UNESP]
dc.contributor.authorFerrisse, Túlio Morandin [UNESP]
dc.contributor.authorJorge, Janaina Habib [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:38:07Z
dc.date.available2022-04-29T08:38:07Z
dc.date.issued2021-12-01
dc.description.abstractThis study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationUnespDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (Unesp), São Paulo
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0261884
dc.identifier.citationPLoS ONE, v. 16, n. 12 December, 2021.
dc.identifier.doi10.1371/journal.pone.0261884
dc.identifier.issn1932-6203
dc.identifier.scopus2-s2.0-85122023317
dc.identifier.urihttp://hdl.handle.net/11449/230139
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleAntimicrobial efficacy and biocompatibility of extracts from Cryptocarya speciesen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.departmentBioquímica e Tecnologia - IQpt

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