Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line

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dc.contributor.authorCardoso, Tereza [UNESP]
dc.contributor.authorTeixeira, Maria C. B. [UNESP]
dc.contributor.authorFachin, Natali [UNESP]
dc.contributor.authorCamargo, Tatiani L.
dc.contributor.authorGomes, Deriane E.
dc.contributor.authorCorte Júnior, Anivaldo O.
dc.contributor.authorAnselmo, Beatriz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:39Z
dc.date.available2014-05-27T11:21:39Z
dc.date.issued2005-10-31
dc.description.abstractInfectious bronchitis virus (IBV) is produced in vitro using specific pathogen free chicken embryos, primary chicken kidney cells (CKC) and/or tracheal organ culture (TOC). Regulatory authorities in Europe (EMEA) and in the United States (FDA) have encouraged biological manufactures to reduce or eliminate the use of live animals and/or products of animal origin in biological manufacturing processes. In this paper, a stable chicken embryo-related (CER) cell line was adapted and maintained in serum free (SF) or animal protein free (APF) medium after a direct switch of the medium. The most suitable media were Ex Cell 520 and Ex Cell 302. CER monolayers adapted to SF or APF were infected with IBV (M41 strain) in agitated suspended culture and the IBV titre obtained 48 h post infection was 2.8 × 104 PFU/ml in both cases. Thus, propagation of CER cells and culture of IBV can be performed without the use of animal serum or animal protein.en
dc.description.affiliationUNESP-Laboratório de Virologia Curso de Medicina Veterinária Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SP
dc.description.affiliationCurso de Mestrado e m Ciência Animal UNESP-Campus de Araçatuba, Br-São Paulo
dc.description.affiliationFellowship Fundação de Amparo a Pesquisa do Estado de São Paulo, Br-São Paulo
dc.description.affiliationFellowship Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq, Br-São Paulo
dc.description.affiliationUNESP-Curso de Medicina Veterinŕia Faculdade de Odontologia Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SP
dc.description.affiliationUnespUNESP-Laboratório de Virologia Curso de Medicina Veterinária Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SP
dc.description.affiliationUnespCurso de Mestrado e m Ciência Animal UNESP-Campus de Araçatuba, Br-São Paulo
dc.description.affiliationUnespUNESP-Curso de Medicina Veterinŕia Faculdade de Odontologia Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent152-156
dc.identifierhttp://www.altex.ch/All-issues/Issue.50.html?iid=76&aid=4
dc.identifier.citationAltex, v. 22, n. 3, p. 152-156, 2005.
dc.identifier.file2-s2.0-27144478971.pdf
dc.identifier.issn0946-7785
dc.identifier.scopus2-s2.0-27144478971
dc.identifier.urihttp://hdl.handle.net/11449/68461
dc.identifier.wosWOS:000232558500005
dc.language.isoeng
dc.relation.ispartofAltex
dc.relation.ispartofsjr1,001
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAnimal protein free media
dc.subjectIBV
dc.subjectSerum free media
dc.subjectVirus production
dc.subjectanimal cell
dc.subjectAvian infectious bronchitis virus
dc.subjectcell line
dc.subjectchicken
dc.subjectcontinuous culture
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectembryo
dc.subjectin vitro study
dc.subjectmonolayer culture
dc.subjectnonhuman
dc.subjectserum
dc.subjectvirus strain
dc.subjectAnimals
dc.subjectBlood Proteins
dc.subjectChick Embryo
dc.subjectCulture Media
dc.subjectCulture Media, Serum-Free
dc.subjectInfectious bronchitis virus
dc.subjectUnited States
dc.subjectUnited States Food and Drug Administration
dc.titleEvaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell lineen
dc.typeArtigo
dcterms.licensehttp://www.altex.ch/About-ALTEX.36.html

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