Cooling of pacu (Piaractus mesopotamicus) embryos at various stages of development for 6 or 10 hours

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dc.contributor.authorLopes, Tais da S. [UNESP]
dc.contributor.authorRomagosa, Elizabeth [UNESP]
dc.contributor.authorStreit, Danilo P. [UNESP]
dc.contributor.authorRibeiro, Ricardo P. [UNESP]
dc.contributor.authorDigmayer, Melanie [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:33:42Z
dc.date.available2014-05-20T15:33:42Z
dc.date.issued2011-02-01
dc.description.abstractThe objective of this research was to verify the effects of cooling embryos of pacu, Piaractus mesopotamicus, in four stages of development during two stocking periods. The stages of embryo development were at: blastoderm, similar to 64 cells-1.4 h after fertilization (haf); 25% of the epiboly movement-5.2 haf; blastoporous closing-8.0 haf; and optical vesicle appearing-13.3 haf. Embryos were exposed to a cryoprotectant solution containing methanol (10%) and sucrose (0.5 M). Thereafter, embryos were submitted to a cooling curve until they reached -8 degrees C, and then kept cooled for 6 or 10 h. In addition, for each stage of embryonic development, a control group with uncooled embryos was used to compare hatching rates. The total number of larvae from the first two stages of ontogenetic development (1.4 and 5.2 haf) was lower compared to the other stages (0.0 and 8.0 haf). There was no significant difference between stages 8.0 and 13.3 haf for the total number of larvae (49.9 +/- 6.7% and 55.2 +/- 6.7%, respectively). Embryo diameter varied according to embryonic stage, providing evidence of differences in membrane permeability. There was a negative correlation between embryo diameter and the total number of larvae (r = -0.372). In conclusion, use of embryonic stages 8.0 and 13.3 haf were recommended for maintaining cooled pacu embryos at -8 degrees C for 6 or 10 h. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Aquaculture Ctr, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aquaculture Ctr, São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPEIXEGEN/Universidade Estadual of Maringa and Aquam/Universidade Federal do Rio Grande do Sul (UFRGS)
dc.description.sponsorshipDUKE Energy Brasil
dc.description.sponsorshipFaculdade de Agronomia Luiz Meneguel
dc.description.sponsorshipLaboratorio de Histologia e Embriologia Animal da Faculdade de Ciencias Agrarias e Veterinaria of Universidade Estadual Paulista
dc.format.extent570-576
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2010.09.030
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 75, n. 3, p. 570-576, 2011.
dc.identifier.doi10.1016/j.theriogenology.2010.09.030
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11449/42253
dc.identifier.wosWOS:000286716400019
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCryopreservationen
dc.subjectOntogenetic developmenten
dc.subjectSouth American fishesen
dc.subjectLarvae survival ratesen
dc.titleCooling of pacu (Piaractus mesopotamicus) embryos at various stages of development for 6 or 10 hoursen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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