High-titer and productivity of l-(+)-lactic acid using exponential fed-batch fermentation with Bacillus coagulans arr4, a new thermotolerant bacterial strain

Coelho, Luciana Fontes [UNESP]; Beitel, Susan Michelz [UNESP]; Sass, Daiane Cristina [UNESP]; Neto, Paulo Marcelo Avila [UNESP]; Contiero, Jonas [UNESP]

High-titer and productivity of l-(+)-lactic acid using exponential fed-batch fermentation with Bacillus coagulans arr4, a new thermotolerant bacterial strain

Arquivos

2-s2.0-85045015311.pdf (246.41 KB)

Data

2018-04-01

Autores

Coelho, Luciana Fontes

Beitel, Susan Michelz

Sass, Daiane Cristina

Neto, Paulo Marcelo Avila

Contiero, Jonas

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Bacillus coagulans arr4 is a thermotolerant microorganism with great biotechnological potential for l-(+)-lactic acid production from granulated sugar and yeast extract. The highest l-(+)-lactic acid production was obtained with Ca(OH)2. The maximum production of l-(+)-lactic acid (206.81 g/L) was observed in exponential feeding using granulated sugar solution (900 g/L) and yeast extract (1%) at 50 °C, pH 6.5, and initial granulated sugar concentration of 100 g/L at 39 h. 5.3 g/L h productivity and 97% yield were observed, and no sugar remained. Comparing the simple batch with exponential fed-batch fermentation, the l(+) lactic acid production was improved in 133.22% and dry cell weight was improved in 83.29%, using granulated sugar and yeast extract. This study presents the highest productivity of lactic acid ever observed in the literature, on the fermentation of thermotolerant Bacillus sp. as well as an innovative and high-efficiency purification technology, using low-cost substances as Celite and charcoal. The recovery of lactic acid was 86%, with 100% protein removal, and the fermentation medium (brown color) became a colorless solution.