High-titer and productivity of l-(+)-lactic acid using exponential fed-batch fermentation with Bacillus coagulans arr4, a new thermotolerant bacterial strain

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dc.contributor.authorCoelho, Luciana Fontes [UNESP]
dc.contributor.authorBeitel, Susan Michelz [UNESP]
dc.contributor.authorSass, Daiane Cristina [UNESP]
dc.contributor.authorNeto, Paulo Marcelo Avila [UNESP]
dc.contributor.authorContiero, Jonas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:19:14Z
dc.date.available2018-12-11T17:19:14Z
dc.date.issued2018-04-01
dc.description.abstractBacillus coagulans arr4 is a thermotolerant microorganism with great biotechnological potential for l-(+)-lactic acid production from granulated sugar and yeast extract. The highest l-(+)-lactic acid production was obtained with Ca(OH)2. The maximum production of l-(+)-lactic acid (206.81 g/L) was observed in exponential feeding using granulated sugar solution (900 g/L) and yeast extract (1%) at 50 °C, pH 6.5, and initial granulated sugar concentration of 100 g/L at 39 h. 5.3 g/L h productivity and 97% yield were observed, and no sugar remained. Comparing the simple batch with exponential fed-batch fermentation, the l(+) lactic acid production was improved in 133.22% and dry cell weight was improved in 83.29%, using granulated sugar and yeast extract. This study presents the highest productivity of lactic acid ever observed in the literature, on the fermentation of thermotolerant Bacillus sp. as well as an innovative and high-efficiency purification technology, using low-cost substances as Celite and charcoal. The recovery of lactic acid was 86%, with 100% protein removal, and the fermentation medium (brown color) became a colorless solution.en
dc.description.affiliationDepartment of Biochemistry and Microbiology Biological Sciences Institute São Paulo State University (UNESP)
dc.description.affiliationSão Paulo State University (UNESP) Bioenergy Research Institute (IPBEN)
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology Biological Sciences Institute São Paulo State University (UNESP)
dc.description.affiliationUnespSão Paulo State University (UNESP) Bioenergy Research Institute (IPBEN)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2010/52416-8
dc.description.sponsorshipIdCNPq: 301567/2012-3
dc.identifierhttp://dx.doi.org/10.1007/s13205-018-1232-0
dc.identifier.citation3 Biotech, v. 8, n. 4, 2018.
dc.identifier.doi10.1007/s13205-018-1232-0
dc.identifier.file2-s2.0-85045015311.pdf
dc.identifier.issn2190-5738
dc.identifier.issn2190-572X
dc.identifier.scopus2-s2.0-85045015311
dc.identifier.urihttp://hdl.handle.net/11449/176144
dc.language.isoeng
dc.relation.ispartof3 Biotech
dc.relation.ispartofsjr0,511
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBacillus sp
dc.subjectFed-batch
dc.subjectl(+) lactic acid
dc.subjectPurification
dc.subjectThermotolerance
dc.titleHigh-titer and productivity of l-(+)-lactic acid using exponential fed-batch fermentation with Bacillus coagulans arr4, a new thermotolerant bacterial strainen
dc.typeArtigo

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