Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen

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dc.contributor.authorVelarde, Johanna Maritza Cano [UNESP]
dc.contributor.authorBastos, Natalia Marins
dc.contributor.authorCarneiro-Leite, Laícia [UNESP]
dc.contributor.authorBorges, Laís Pedroso [UNESP]
dc.contributor.authorVieira, Edson Guilherme [UNESP]
dc.contributor.authorVeríssimo-Silveira, Rosicleire [UNESP]
dc.contributor.authorNinhaus-Silveira, Alexandre [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:43:52Z
dc.date.available2023-07-29T13:43:52Z
dc.date.issued2023-01-01
dc.description.abstractThis study aimed to develop a protocol for the cryopreservation of Pseudoplatystoma corruscans semen. For this, mature males were hormonally induced with a single dose of carp pituitary extract (5 mg/kg body weight). Semen was collected and evaluated. Two cryoprotectants were tested to compose the diluents: dimethyl acetamide (DMA) and dimethyl sulfoxide (Me2 SO), in two concentrations (8% and 10%), + 5.0% glucose + 10% egg yolk. The semen was diluted in a 1: 4 ratio (semen: extender), packed in 0.5 mL straws and frozen in a dry shipper container in liquid nitrogen vapors. After thawing, sperm kinetics, sperm morphology and DNA integrity of cryopreserved sperm were evaluated. Pseudoplatystoma corruscans males produced semen with sperm motility > 80%. After thawing, all treatments provided semen with total sperm motility > 40%, with no significant difference (P < 0.05) between them, as well as between the other sperm kinetic parameters evaluated. The treatments with DMA provided a smaller fragmentation of the DNA of the gametes. Sperm malformations were identified in both fresh and cryopreserved semen, with a slight increase in these malformations being identified in sperm from thawed P. corruscans semen samples.en
dc.description.affiliationPós-Graduação em Biologia Animal Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Cristóvão Colombo, 2265, Jardim Nazareth, SP
dc.description.affiliationPós-Graduação em Biociência Animal Faculdade de Zootecnia e Engenharia de Alimentos (FZEA – USP), Rua Duque de Caxias, 225, Jardim Elite, SP
dc.description.affiliationPós-Graduação em Ciências Biológicas Zoologia Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Av. Rubião Jr., s/n, SP
dc.description.affiliationLaboratório de Ictiologia Neotropical (L.I.NEO) Departamento de Biologia e Zootecnia Faculdade de Engenharia UNESP, Av. Brasil, 56, SP
dc.description.affiliationUnespPós-Graduação em Biologia Animal Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Cristóvão Colombo, 2265, Jardim Nazareth, SP
dc.description.affiliationUnespPós-Graduação em Ciências Biológicas Zoologia Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Av. Rubião Jr., s/n, SP
dc.description.affiliationUnespLaboratório de Ictiologia Neotropical (L.I.NEO) Departamento de Biologia e Zootecnia Faculdade de Engenharia UNESP, Av. Brasil, 56, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/12522–9
dc.identifierhttp://dx.doi.org/10.1590/1982-0224-2022-0071
dc.identifier.citationNeotropical Ichthyology, v. 21, n. 1, 2023.
dc.identifier.doi10.1590/1982-0224-2022-0071
dc.identifier.issn1982-0224
dc.identifier.issn1679-6225
dc.identifier.scopus2-s2.0-85149048745
dc.identifier.urihttp://hdl.handle.net/11449/248432
dc.language.isoeng
dc.relation.ispartofNeotropical Ichthyology
dc.sourceScopus
dc.subjectDNA integrity
dc.subjectFreezing
dc.subjectGermplasm
dc.subjectPimelodidae
dc.subjectSperm quality
dc.titleDimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semenen
dc.typeArtigo

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