Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen

Velarde, Johanna Maritza Cano [UNESP]; Bastos, Natalia Marins; Carneiro-Leite, Laícia [UNESP]; Borges, Laís Pedroso [UNESP]; Vieira, Edson Guilherme [UNESP]; Veríssimo-Silveira, Rosicleire [UNESP]; Ninhaus-Silveira, Alexandre [UNESP]

Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen

Data

2023-01-01

Autores

Velarde, Johanna Maritza Cano

Bastos, Natalia Marins

Carneiro-Leite, Laícia

Borges, Laís Pedroso

Vieira, Edson Guilherme

Veríssimo-Silveira, Rosicleire

Ninhaus-Silveira, Alexandre

Tipo

Artigo

Resumo

This study aimed to develop a protocol for the cryopreservation of Pseudoplatystoma corruscans semen. For this, mature males were hormonally induced with a single dose of carp pituitary extract (5 mg/kg body weight). Semen was collected and evaluated. Two cryoprotectants were tested to compose the diluents: dimethyl acetamide (DMA) and dimethyl sulfoxide (Me2 SO), in two concentrations (8% and 10%), + 5.0% glucose + 10% egg yolk. The semen was diluted in a 1: 4 ratio (semen: extender), packed in 0.5 mL straws and frozen in a dry shipper container in liquid nitrogen vapors. After thawing, sperm kinetics, sperm morphology and DNA integrity of cryopreserved sperm were evaluated. Pseudoplatystoma corruscans males produced semen with sperm motility > 80%. After thawing, all treatments provided semen with total sperm motility > 40%, with no significant difference (P < 0.05) between them, as well as between the other sperm kinetic parameters evaluated. The treatments with DMA provided a smaller fragmentation of the DNA of the gametes. Sperm malformations were identified in both fresh and cryopreserved semen, with a slight increase in these malformations being identified in sperm from thawed P. corruscans semen samples.