Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds

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dc.contributor.authorCavada, B. S.
dc.contributor.authorMarinho, E. S.
dc.contributor.authorSouza, E. P.
dc.contributor.authorBenevides, R. G.
dc.contributor.authorDelatorre, P.
dc.contributor.authorSouza, LAG
dc.contributor.authorNascimento, K. S.
dc.contributor.authorSampaio, A. H.
dc.contributor.authorMoreno, FBMB
dc.contributor.authorRustiguel, JKR
dc.contributor.authorCanduri, F.
dc.contributor.authorde Azevedo, W. F.
dc.contributor.authorDebray, H.
dc.contributor.institutionUniversidade Federal do Ceará (UFC)
dc.contributor.institutionInstituto Nacional de Pesquisas da Amazônia (INPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionPontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
dc.contributor.institutionUniv Sci & Technol Lille
dc.date.accessioned2014-05-20T15:20:22Z
dc.date.available2014-05-20T15:20:22Z
dc.date.issued2006-03-01
dc.description.abstractA lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8% (w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 angstrom resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress.en
dc.description.affiliationFed Univ Ceara, Dept Biochem, BioMol Lab, Fortaleza, Ceara, Brazil
dc.description.affiliationINPA, Manaus, Amazonas, Brazil
dc.description.affiliationFed Univ Ceara, Biomol Mat Fishing Engn Fac, Fortaleza, Ceara, Brazil
dc.description.affiliationUNESP, Dept Fis, Programa Posgrad Biofis Mol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUFMS, CCBS, Dept Morfofisiol, BR-79070900 Campo Grande, MS, Brazil
dc.description.affiliationPUCRS, Fac Biociencias, BR-90619900 Porto Alegre, RS, Brazil
dc.description.affiliationUniv Sci & Technol Lille, CNRS, UMR 8576, Lille, France
dc.description.affiliationUniv Sci & Technol Lille, Chim Biol Lab, Lille, France
dc.description.affiliationUnespUNESP, Dept Fis, Programa Posgrad Biofis Mol, Sao Jose do Rio Preto, SP, Brazil
dc.format.extent235-237
dc.identifierhttp://dx.doi.org/10.1107/S174430910600371X
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 235-237, 2006.
dc.identifier.doi10.1107/S174430910600371X
dc.identifier.issn1744-3091
dc.identifier.urihttp://hdl.handle.net/11449/31680
dc.identifier.wosWOS:000235681500016
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titlePurification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seedsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Física - IBILCE