In vitro Paracoccidioides brasiliensis biofilm and gene expression of adhesins and hydrolytic enzymes

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dc.contributor.authorOrlandi Sardi, Janaina de Cassia [UNESP]
dc.contributor.authorPitangui, Nayla de Souza [UNESP]
dc.contributor.authorVoltan, Aline Raquel [UNESP]
dc.contributor.authorBraz, Jaqueline Derissi [UNESP]
dc.contributor.authorMachado, Marcelo Pelajo
dc.contributor.authorFusco Almeida, Ana Marisa [UNESP]
dc.contributor.authorSoares Mendes Giannini, Maria Jose [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Oswaldo Cruz
dc.date.accessioned2018-11-26T16:17:29Z
dc.date.available2018-11-26T16:17:29Z
dc.date.issued2015-08-18
dc.description.abstractParacoccidioides species are dimorphic fungi that initially infect the lungs but can also spread throughout the body. The spreading infection is most likely due to the formation of a biofilm that makes it difficult for the host to eliminate the infection. Biofilm formation is crucial for the development of infections and confines the pathogen to an extracellular matrix. Its presence is associated with antimicrobial resistance and avoidance of host defenses. This current study provides the first description of biofilm formation by Paracoccidioides brasiliensis (Pb18) and an analysis of gene expression, using real-time PCR, associated with 3 adhesins and 2 hydrolytic enzymes that could be associated with the virulence profile. Biofilm formation was analyzed using fluorescence microscopy, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Metabolic activity was determined using the XTT reduction assay. P. brasiliensis was able to form mature biofilm in 144h with a thickness of 100m. The presence of a biofilm was found to be associated with an increase in the expression of adhesins and enzymes. GP43, enolase, GAPDH and aspartyl proteinase genes were over-expressed, whereas phospholipase was down-regulated in biofilm. The characterization of biofilm formed by P. brasiliensis may contribute to a better understanding of the pathogenesis of paracoccidioidomycosis as well as the search for new therapeutic alternatives; while improving the effectiveness of treatment.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Micol Clin, Dept Anal Clin, Araraquara, Brazil
dc.description.affiliationInst Oswaldo Cruz, Dept Patol, BR-20001 Rio De Janeiro, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Micol Clin, Dept Anal Clin, Araraquara, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPADC (Support Program for Scientific, Faculty of Pharmaceutical Sciences, UNESP)
dc.format.extent663-672
dc.identifierhttp://dx.doi.org/10.1080/21505594.2015.1031437
dc.identifier.citationVirulence. Philadelphia: Taylor & Francis Inc, v. 6, n. 6, p. 663-672, 2015.
dc.identifier.doi10.1080/21505594.2015.1031437
dc.identifier.fileWOS000364945600019.pdf
dc.identifier.issn2150-5594
dc.identifier.urihttp://hdl.handle.net/11449/160978
dc.identifier.wosWOS:000364945600019
dc.language.isoeng
dc.publisherTaylor & Francis Inc
dc.relation.ispartofVirulence
dc.relation.ispartofsjr1,635
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectadhesins
dc.subjectbiofilms
dc.subjectgene expression
dc.subjectParacoccidioides brasiliensis
dc.subjectreal-time PCR
dc.subjectvirulence factors
dc.titleIn vitro Paracoccidioides brasiliensis biofilm and gene expression of adhesins and hydrolytic enzymesen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Inc
unesp.author.lattes3716273524139678[6]
unesp.author.orcid0000-0002-2115-8988[6]
unesp.departmentAnálises Clínicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas