Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas

dc.contributor.authorda Silva, Adriano Bortolotti
dc.contributor.authordos Reis, Caroline Oliveira
dc.contributor.authorCazetta, Jairo Osvaldo [UNESP]
dc.contributor.authorCarlin, Samira Domingues
dc.contributor.authorLandgraf, Paulo Roberto Corrêa
dc.contributor.authordos Reis, Marinacunha
dc.contributor.institutionUNIFENAS
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUPD
dc.date.accessioned2022-04-29T07:35:50Z
dc.date.available2022-04-29T07:35:50Z
dc.date.issued2016-05-01
dc.description.abstractMicropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants.en
dc.description.affiliationFaculdade de Agronomia da Universidade José do Rosário Vellano UNIFENAS
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Tecnologia
dc.description.affiliationAgência Paulista de Tecnologia dos Agronegócios Pólo Regional Centro Oeste UPD
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Tecnologia
dc.format.extent619-626
dc.identifierhttp://dx.doi.org/10.14393/BJ-v32n3a2016-31368
dc.identifier.citationBioscience Journal, v. 32, n. 3, p. 619-626, 2016.
dc.identifier.doi10.14393/BJ-v32n3a2016-31368
dc.identifier.issn1981-3163
dc.identifier.issn1516-3725
dc.identifier.scopus2-s2.0-84971607201
dc.identifier.urihttp://hdl.handle.net/11449/228158
dc.language.isoeng
dc.relation.ispartofBioscience Journal
dc.sourceScopus
dc.subjectAbiotic stress
dc.subjectCattleya walkeriana
dc.subjectMicropropagation
dc.titleEfeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeaspt
dc.title.alternativeEffects of exogenous proline and a natural ventilation system on the in vitro growth of orchidsen
dc.typeArtigo
unesp.departmentTecnologia - FCAVpt

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