Graphene oxide-based nanomaterial interaction with human breast cancer cells

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dc.contributor.authorRibeiro, Beatriz F. M.
dc.contributor.authorSouza, Marcelo M.
dc.contributor.authorFernandes, Daniela S. [UNESP]
dc.contributor.authorCarmo, Devaney R. do [UNESP]
dc.contributor.authorMachado-Santelli, Glaucia M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFac Philosophy Sci & Arts Ribeirao Preto
dc.date.accessioned2020-12-10T17:03:53Z
dc.date.available2020-12-10T17:03:53Z
dc.date.issued2019-12-27
dc.description.abstractGraphene and graphene-based nanomaterials have great potential for various biomedical applications due to their unique physicochemical properties. However, how graphene-based nanomaterials interact with biological systems has not been thoroughly studied. This study shows that 24, 48, and 72 hr exposure of 2.4 mu g/cm(2) of graphene oxide (GOX) and GOX modified with DAB-AM-16 and PAMAM dendrimers (GOXD and GOXP, respectively) did not exhibit toxicity to MCF-7 cells. However, higher graphene concentrations, such as 24 and 48 mu g/cm(2), induced low cytotoxic effects. The GOX, GOXD, and GOXP particles have a strong affinity with the cellular membrane. Cells that internalized the nanomaterials presented morphological alterations and modifications in the organization of microfilaments and microtubules compared with control cells. Then, cells were treated with 24 mu g/cm(2) of GOX, GOXD or GOXP for 24 hr and recovered for an additional period of 24 hr in normal medium. Nanoparticles remained in the cytoplasm of some cells, apparently with no effect on cellular morphology, being consistent with the data found in the cell proliferation experiment, which showed that the cells remained alive up to 72 hr.en
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Phys & Chem, Sao Paulo, SP, Brazil
dc.description.affiliationFac Philosophy Sci & Arts Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Phys & Chem, Sao Paulo, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 303464/2017-8
dc.description.sponsorshipIdFAPESP: 2015/20397-8
dc.description.sponsorshipIdFAPESP: 2017/16893-5
dc.description.sponsorshipIdFAPESP: 2018/22882-9
dc.format.extent863-870
dc.identifierhttp://dx.doi.org/10.1002/jbm.a.36864
dc.identifier.citationJournal Of Biomedical Materials Research Part A. Hoboken: Wiley, v. 108, n. 4, p. 863-870, 2020.
dc.identifier.doi10.1002/jbm.a.36864
dc.identifier.issn1549-3296
dc.identifier.urihttp://hdl.handle.net/11449/195078
dc.identifier.wosWOS:000504509500001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Biomedical Materials Research Part A
dc.sourceWeb of Science
dc.subjectcell morphology
dc.subjectcell proliferation
dc.subjectDendrimer
dc.subjectGraphene oxide
dc.subjectnano-bio interaction
dc.titleGraphene oxide-based nanomaterial interaction with human breast cancer cellsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell

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