The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome

Página do item simplificado
dc.contributor.authorCamargo, A. A.
dc.contributor.authorSamaia, HPB
dc.contributor.authorDias-Neto, E.
dc.contributor.authorSimao, D. F.
dc.contributor.authorMigotto, I. A.
dc.contributor.authorBriones, MRS
dc.contributor.authorCosta, F. F.
dc.contributor.authorNagai, M. A.
dc.contributor.authorVerjovski-Almeida, S.
dc.contributor.authorZago, M. A.
dc.contributor.authorAndrade, LEC
dc.contributor.authorCarrer, H.
dc.contributor.authorEl-Dorry, HFA
dc.contributor.authorEspreafico, E. M.
dc.contributor.authorHabr-Gama, A.
dc.contributor.authorGiannella-Neto, D.
dc.contributor.authorGoldman, G. H.
dc.contributor.authorGruber, A.
dc.contributor.authorHackel, C.
dc.contributor.authorKimura, E. T.
dc.contributor.authorMaciel, RMB
dc.contributor.authorMarie, SKN
dc.contributor.authorMartins, EAL
dc.contributor.authorNobrega, M. P.
dc.contributor.authorPaco-Larson, M. L.
dc.contributor.authorPardini, MIMC
dc.contributor.authorPereira, G. G.
dc.contributor.authorPesquero, J. B.
dc.contributor.authorRodrigues, V
dc.contributor.authorRogatto, Silvia Regina [UNESP]
dc.contributor.authorda Silva, IDCG
dc.contributor.authorSogayar, M. C.
dc.contributor.authorSonati, MDF
dc.contributor.authorTajara, E. H.
dc.contributor.authorValentini, Sandro Roberto [UNESP]
dc.contributor.authorAlberto, F. L.
dc.contributor.authorAmaral, MEJ
dc.contributor.authorAneas, I
dc.contributor.authorArnaldi, LAT
dc.contributor.authorde Assis, A. M.
dc.contributor.authorBengtson, M. H.
dc.contributor.authorBergamo, N. A.
dc.contributor.authorBombonato, V
dc.contributor.authorde Camargo, MER
dc.contributor.authorCanevari, R. A.
dc.contributor.authorCarraro, D. M.
dc.contributor.authorCerutti, J. M.
dc.contributor.authorCorrea, MLC
dc.contributor.authorCorrea, RFR
dc.contributor.authorCosta, MCR
dc.contributor.authorCurcio, C.
dc.contributor.authorHokama, POM
dc.contributor.authorFerreira, AJS
dc.contributor.authorFuruzawa, G. K.
dc.contributor.authorGushiken, T.
dc.contributor.authorHo, P. L.
dc.contributor.authorKimura, E.
dc.contributor.authorKrieger, J. E.
dc.contributor.authorLeite, LCC
dc.contributor.authorMajumder, P.
dc.contributor.authorMarins, M.
dc.contributor.authorMarques, E. R.
dc.contributor.authorMelo, ASA
dc.contributor.authorMelo, M.
dc.contributor.authorMestriner, C. A.
dc.contributor.authorMiracca, E. C.
dc.contributor.authorMiranda, D. C.
dc.contributor.authorNascimento, ALTO
dc.contributor.authorNobrega, F. G.
dc.contributor.authorOjopi, EPB
dc.contributor.authorPandolfi, JRC
dc.contributor.authorPessoa, L. G.
dc.contributor.authorPrevedel, A. C.
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorRainho, C. A.
dc.contributor.authorReis, EMR
dc.contributor.authorRibeiro, M. L.
dc.contributor.authorda Ros, N.
dc.contributor.authorde Sa, R. G.
dc.contributor.authorSales, M. M.
dc.contributor.authorSant'anna, S. C.
dc.contributor.authordos Santos, M. L.
dc.contributor.authorda Silva, A. M.
dc.contributor.authorda Silva, N. P.
dc.contributor.authorSilva, W. A.
dc.contributor.authorda Silveira, R. A.
dc.contributor.authorSousa, J. F.
dc.contributor.authorStecconi, D.
dc.contributor.authorTsukumo, F.
dc.contributor.authorValente, Valeria [UNESP]
dc.contributor.authorSoares, F.
dc.contributor.authorMoreira, E. S.
dc.contributor.authorNunes, D. N.
dc.contributor.authorCorrea, R. G.
dc.contributor.authorZalcberg, H.
dc.contributor.authorCarvalho, A. F.
dc.contributor.authorReis, LFL
dc.contributor.authorBrentani, R. R.
dc.contributor.authorSimpson, AJG
dc.contributor.authorde Souza, S. J.
dc.contributor.institutionLudwig Inst Canc Res
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniv Vale Paraiba
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHops AC Camargo
dc.date.accessioned2014-05-20T13:24:37Z
dc.date.available2014-05-20T13:24:37Z
dc.date.issued2001-10-09
dc.description.abstractopen reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency of the strategy and its potential contribution to the definition of the human transcriptome. We estimate that ORESTES sampled over 80% of all highly and moderately expressed, and between 40% and 50% of rarely expressed, human genes. In our most thoroughly sequenced tissue, the breast, the 130,000 ORESTES generated are derived from transcripts from an estimated 70% of all genes expressed in that tissue, with an equally efficient representation of both highly and poorly expressed genes. In this respect, we find that the capacity of the ORESTES strategy both for gene discovery and shotgun transcript sequence generation significantly exceeds that of conventional ESTs. The distribution of ORESTES is such that many human transcripts are now represented by a scaffold of partial sequences distributed along the length of each gene product. The experimental joining of the scaffold components, by reverse transcription-PCR, represents a direct route to transcript finishing that may represent a useful alternative to full-length cDNA cloning.en
dc.description.affiliationLudwig Inst Canc Res, BR-01509010 São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, UNIFESP, Dept Reumatol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, UNIFESP, Dept Biofis, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, UNIFESP, Escola Paulista Med, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Hemoctr, BR-13089970 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Radiol, BR-01296903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05513970 São Paulo, Brazil
dc.description.affiliationFac Med Ribeirao Preto, Dept Clin Med, BR-14049900 São Paulo, Brazil
dc.description.affiliationFac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Pathogenicos, BR-14049900 São Paulo, Brazil
dc.description.affiliationFac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciências Biol, BR-13418900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Inst Coracao, INCOR, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Lab Nutr & Doencas Metab, BR-01246 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Neurol, BR-01246 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Ciências Farmaceut Ribeirao Preto, Dept Ciências Farmaceut, BR-14040903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Vet & Zootecn, Dept Patol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Ciências Med, Dept Med Genet, BR-13081970 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciências Biomed, Dept Histol Embriol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Med, BR-04029032 São Paulo, Brazil
dc.description.affiliationInst Butantan, Ctr Biotecnol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv Vale Paraiba, Inst Pesquisa & Desenvolvimento, BR-12244 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Botucatu, Hemoctr, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Ciências Med, Inst Biol, Dept Genet & Evolucao, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Ciências Med, Dept Patol Clin, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Genet, BR-18618000 São Paulo, Brazil
dc.description.affiliationEscola Paulista Med, Dept Ginecol & Obstet, BR-04301900 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exatas, Dept Biol, BR-15054 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Farmaceut & Araraquara, Dept Ciências Biol, BR-14801902 São Paulo, Brazil
dc.description.affiliationHops AC Camargo, Dept Anat Patol, BR-01509010 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Botucatu, Hemoctr, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Genet, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exatas, Dept Biol, BR-15054 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Farmaceut & Araraquara, Dept Ciências Biol, BR-14801902 São Paulo, Brazil
dc.format.extent12103-12108
dc.identifierhttp://dx.doi.org/10.1073/pnas.201182798
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 98, n. 21, p. 12103-12108, 2001.
dc.identifier.doi10.1073/pnas.201182798
dc.identifier.issn0027-8424
dc.identifier.lattes5333250355049814
dc.identifier.lattes5050653153437898
dc.identifier.lattes8173733141422929
dc.identifier.lattes7991082362671212
dc.identifier.lattes8814823545159504
dc.identifier.orcid0000-0002-3942-7744
dc.identifier.orcid0000-0003-3474-4422
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.orcid0000-0002-0285-1162
dc.identifier.urihttp://hdl.handle.net/11449/7701
dc.identifier.wosWOS:000171558900052
dc.language.isoeng
dc.publisherNatl Acad Sciences
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.relation.ispartofjcr9.504
dc.relation.ispartofsjr6,092
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleThe contribution of 700,000 ORF sequence tags to the definition of the human transcriptomeen
dc.typeArtigo
dcterms.licensehttp://www.pnas.org/site/misc/authorlicense.pdf
dcterms.rightsHolderNatl Acad Sciences
unesp.author.lattes5333250355049814
unesp.author.lattes5050653153437898
unesp.author.lattes8173733141422929[52]
unesp.author.lattes7991082362671212[74]
unesp.author.lattes8814823545159504[75]
unesp.author.orcid0000-0002-3942-7744[90]
unesp.author.orcid0000-0003-3474-4422[52]
unesp.author.orcid0000-0001-5693-6148[74]
unesp.author.orcid0000-0002-0285-1162[75]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Araraquara - FCF - Faculdade de Ciências Farmacêuticas