Peripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain

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dc.contributor.authorBrigatte, Patricia [UNESP]
dc.contributor.authorKonno, Katsuhiro
dc.contributor.authorGutierrez, Vanessa Pacciari
dc.contributor.authorSampaio, Sandra Coccuzzo
dc.contributor.authorZambelli, Vanessa Olzon
dc.contributor.authorPicolo, Gisele
dc.contributor.authorCuri, Rui
dc.contributor.authorCury, Yara
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Toyama
dc.date.accessioned2014-05-27T11:29:41Z
dc.date.available2014-05-27T11:29:41Z
dc.date.issued2013-06-17
dc.description.abstractCancer pain is an important clinical problem and may not respond satisfactorily to the current analgesic therapy. We have characterized a novel and potent analgesic peptide, crotalphine, from the venom of the South American rattlesnake Crotalus durissus terrificus. In the present work, the antinociceptive effect of crotalphine was evaluated in a rat model of cancer pain induced by intraplantar injection of Walker 256 carcinoma cells. Intraplantar injection of tumor cells caused the development of hyperalgesia and allodynia, detected on day 5 after tumor cell inoculation. Crotalphine (6 μg/kg), administered p.o., blocked both phenomena. The antinociceptive effect was detected 1 h after treatment and lasted for up to 48 h. Intraplantar injection of nor-binaltorphimine (50 g/paw), a selective antagonist of κ-opioid receptors, antagonized the antinociceptive effect of the peptide, whereas N,N-diallyl-Tyr-Aib-Phe-Leu (ICI 174,864, 10 μg/paw), a selective antagonist of δ-opioid receptors, partially reversed this effect. On the other hand, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr amide (CTOP, 20 g/paw), an antagonist of μ-opioid receptors, did not modify crotalphine-induced antinociception. These data indicate that crotalphine induces a potent and long lasting opioid-mediated antinociception in cancer pain. © 2013 Elsevier Inc.en
dc.description.affiliationLaboratório Especial de Dor e Sinalização Laboratório de Fisiopatologia Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 Sao Paulo, SP
dc.description.affiliationCentro de Toxinologia Aplicada Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 Sao Paulo, SP
dc.description.affiliationDepartamento de Fisiologia e Biofísica Instituto de Ciências Biomédicas Universidade de São Paulo, Av. Prof. Lineu Prestes, 1524, 05508-900 Sao Paulo, SP
dc.description.affiliationDepartamento de Biologia Instituto de Biociências de Rio Claro Universidade Estadual Paulista (UNESP), Rio Claro, SP
dc.description.affiliationInstitute of Natural Medicine University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 930-0194
dc.description.affiliationUnespDepartamento de Biologia Instituto de Biociências de Rio Claro Universidade Estadual Paulista (UNESP), Rio Claro, SP
dc.format.extent1-7
dc.identifierhttp://dx.doi.org/10.1016/j.pbb.2013.04.012
dc.identifier.citationPharmacology Biochemistry and Behavior, v. 109, p. 1-7.
dc.identifier.doi10.1016/j.pbb.2013.04.012
dc.identifier.issn0091-3057
dc.identifier.issn1873-5177
dc.identifier.scopus2-s2.0-84878835594
dc.identifier.urihttp://hdl.handle.net/11449/75669
dc.language.isoeng
dc.relation.ispartofPharmacology Biochemistry and Behavior
dc.relation.ispartofjcr2.538
dc.relation.ispartofsjr1,150
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAntinociception
dc.subjectCancer pain
dc.subjectCrotalphine
dc.subjectOpioid receptors
dc.subjectWalker 256 carcinoma cells
dc.subjectantinociceptive agent
dc.subjectcrotalphine
dc.subjectdelta opiate receptor
dc.subjectkappa opiate receptor
dc.subjectmorphine
dc.subjectunclassified drug
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectantinociception
dc.subjectcancer growth
dc.subjectcancer pain
dc.subjectdrug effect
dc.subjectdrug efficacy
dc.subjectlocomotion
dc.subjectmale
dc.subjectnonhuman
dc.subjectpain threshold
dc.subjectpriority journal
dc.subjectrat
dc.titlePeripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer painen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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